CRPS
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB44143 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR5950070_1.fastq.gz | Fastqsanger.gz | |||
| ERR5950070_2.fastq.gz | Fastqsanger.gz | |||
| ERR5950080_1.fastq.gz | Fastqsanger.gz | |||
| ERR5950080_2.fastq.gz | Fastqsanger.gz | |||
| ERR5950090_1.fastq.gz | Fastqsanger.gz |
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Project description:Identification of Two Biological Subgroups of Complex Regional Pain Syndrome Type 1 by Transcriptomic Profiling of Skin and Blood
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Project description:Chronic neuropathic pain is widespread, but individual diagnostic biomarkers are unknown and primate specificity hampers rodent validation tests. Here, we report that microRNA (miR)-sequencing of blood leukocytes from 250 multi-centre pain patients revealed complex regional pain syndrome (CRPS)-related increases in the pain-related microRNA (miR)-21-5p and decreases in the cholinergic-targeted miR-335-5p. Both these miRs were similarly modified in dorsal root ganglia of nerve-injured mice, where long RNA-sequencing detected pain-associated elevation of neurogenesis and inflammation-related transcripts. Moreover, either genomic ablation or intrathecal antisense oligonucleotide neuro-suppression of miR-21a-5p, but not genetic debilitation of B7-H1 lymphocytes alleviated murine pain responses while reducing cellular pathways that are induced in patients’ leukocytes; and a cooperative leukocyte ‘signature’ of 12 miRs including miR-21a-5p and -335-5p predicted 96% of CRPS symptoms severity. Our findings implicate cholinergic-suppressible neuro-inflammation processes in chronic acute pain, suggest pathway-based cooperative validation tests and open unprecedented options for mechanistic studies of chronic pain disorders.
2025-08-31 | GSE106837 | GEO
Project description:Complex regional pain syndrome type-I (CRPS-I) is chronic neurological disorder accompanied with devastating pain. In this study, we employed single-cell sequencing technology to analyze the ipsilateral L4-L6 dorsal root ganglia in both Sham and CPIP groups, aiming to investigate the mechanisms underlying chronic pain development in the CPIP model.
2025-04-14 | GSE294564 | GEO
Project description:Complex regional pain syndrome type-I (CRPS-I) is chronic neurological disorder accompanied with devastating pain. Most conventional medical treatments lack effectiveness, making CRPS-I a challenging clinical condition. Electroacupuncture (EA) showed effectiveness in alleviating the pain symptoms of CRPS-I patients. However, the molecular mechanisms underlying EA’s therapeutic effect are still not well understood. Here, we established the rat chronic post-ischemic pain (CPIP) model to mimic CRPS-I and performed repetitive EA on bilateral hind limbs of the CPIP model rats. We then performed RNA-sequencing (RNA-Seq) to study the differences in gene expression, gene networks and molecular pathways in ipsilateral DRGs innervating the hind limb of the CPIP model rats with and without repetitive EA treatment. Our results found that repetitive EA treatment significantly alleviated mechanical allodynia in bilateral hind limbs of CPIP model rats. RNA-Seq analysis indicated that EA modulated the expression of multiple genes and gene networks in the DRGs of CPIP model rats. Further bioinformatics analysis identified the up-regulation of an array of genes involved in biological process such as neutrophil chemotaxis and immune response in the DRGs of CPIP model rats after EA treatment. Thus, these results suggest that EA may alleviate pain response in CPIP model rats via regulating multiple genes. Our work may help to further advance the understandings of the molecular mechanisms underlying EA’s therapeutic effects on CRPS-I and help to identify novel targets for CRPS-I treatment
2020-09-26 | GSE158560 | GEO
Project description:Genome-wide expression profiling of complex regional pain syndrome
| PRJNA206506 | ENA
Project description:we successfully identified pain-related genes through genome-wide expression profiling in blood from CRPS patients. We found that 80 genes were differentially expressed between 4 CRPS patients (2 CRPS I and 2 CRPS II) and 5 controls (cut-off value: 1.5-fold change and p < 0.05). Most of those genes were associated with signal transduction, developmental processes, cell structure and motility, and immunity and defense. The expression levels of 12 genes selected from the microarray were confirmed in 24 CRPS patients and 18 controls by quantitative reverse transcription-polymerase chain reaction. We focused on matrix metalloproteinase 9 (MMP9) that showed statistically significant difference from controls and the highest relative fold change in CRPS II patients to controls by qPCR validation (6.4 ± 2.47 times and p = 0.001). Identification of pain-related genes in peripheral blood of complex regional pain syndrome (CRPS) using cDNA microarray. Comparative gene expression profiles in peripheral blood of 5 healthy controls and 4 CRPS patients.
2013-06-06 | E-GEOD-47603 | biostudies-arrayexpress
Project description:Inflammation may lead to pain, but the underlying mechanisms remain unclear. Here, we identify cooperating small non-coding RNA (sncRNA) signatures including microRNAs (miRs) and transfer RNA fragments (tRFs) that may drive inflammatory pain in both acute and chronic settings. Initiating lipopolysaccharide (LPS)-induced inflammation altered ganglion-specific, time-dependent miRs and tRFs in murine trigeminal (sensory) and superior cervical (sympathetic) ganglia. Indicating conserved pain-related regulatory elements, many of those changes overlapped with those observed in fibromyalgia syndrome (FMS) patient-derived blood cells and keratinocytes. Moreover, cross-species integrative analyses revealed two oppositely correlated sncRNA clusters whose expression ratios distinguished inflammatory from non-inflammatory states and increased during human nociceptor differentiation. Further suggesting functional roles in pain modulation, these pain-related sncRNAs targeted distinct long RNAs expressed in pain- and cholinergic-related states. Importantly, these sncRNAs and their associated transcripts were validated in datasets from both a murine spinal nerve injury model and synovium samples from osteoarthritis patients with varying pain intensities. Our findings reveal that specific miRs and tRFs act as conserved regulators of nociceptive signaling across species, tissues and pain modalities. Further, this work reveals new understanding of the molecular underpinnings of pain and highlights the notion that mammalian pain- and cholinergic-related sncRNAs actively contribute to the shift from inflammation to peripheral nervous system pain, including fibromyalgia pain.
2025-08-24 | GSE303093 | GEO
Project description:We used microarray-based expression genomics in 25 inbred mouse strains to identify dorsal root ganglion (DRG)-expressed genetic contributors to mechanical allodynia a prominent symptom of chronic pain. Expression genetics identifies a role for the Chrna6 (alpha 6-nicotinic receptor) gene in pain in mice and humans. Dorsal root ganglion tissue across multiple inbred mouse strains, both male and female
2015-02-18 | E-GEOD-65997 | biostudies-arrayexpress
Project description:Background & Aims: Irritable bowel syndrome (IBS) is a disorder characterized by chronic abdominal pain and is linked to post-inflammatory and stress-correlated factors that cause changes in the perception of visceral events. Increased evidence indicates that probiotic bacteria may be useful in treating IBS. Our aims were to evaluate the efficacy of treatment with VSL#3, a mixture of 8 probiotic bacteria strains, in the neonatal maternal separation (NMS)-induced visceral hypersensitivity rat model and to determine whether it modulates the colonic expression of pain-related genes. Methods: Male NMS pups were treated orally with placebo or VSL#3 at days 3-60, while normal, not separated rats were used as control. After 60 days from birth, perception of painful sensation induced by colorectal distension (CRD) was measured by assessing the abdominal withdrawal reflex (score 0-4). The colonic gene expression analysis was assessed by using Agilent Whole Rat Genome Oligo Microarrays. Results: NMS rats exhibited both hyperalgesia and allodynia when compared with controls. VSL#3 showed a potent analgesic effect on CRD-induced pain without modifying colorectal compliance. The microarray analysis demonstrated that NMS rats had both over- and downregulation of several genes involved in inflammatory and painful processes and VSL#3 was able to counteract these alterations. Conclusions: This study indicates that VSL#3 is effective in reducing visceral pain in an experimental model of IBS by induction or suppression of pain-modulating genes. These observations provide support for the use of VSL#3 in the treatment of painful conditions related to IBS. The dataset comprises 12 samples divided into three sample groups each representing a certain treatment condition of male rats.
2013-06-26 | E-GEOD-38942 | biostudies-arrayexpress