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ABSTRACT:
PROVIDER: PRJEB44558 | ENA |
REPOSITORIES: ENA
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Project description:We investigated the evolution of sperm conjugation in phylum Arthropoda by reconstructing its diversification along a time-calibrated phylogeny for 621 arthropods and related ecdysozoans. Phylogenetic modeling reveals conjugation has had a dynamic evolution history with multiple origins of all five observed categorical types of sperm conjugates. Contrary to prior predictions that conjugation is taxonomically widespread but relatively rare within the clades in which it is observed, our reconstructions suggest conjugation is pervasive in the arthropods, whose tree is estimated to have had conjugated sperm for 40% of its time. Conjugation has likely evolved between 39-56 times independently, and our results indicate aggregates were likely present in an early hexapod ancestor nearly 500 million years before the present. Specialized conjugate types such as spermatostyles are estimated to have been derived independently multiple times, including in both the true bugs (Order Hemiptera) and beetles (Order Coleoptera). We leverage spermatostyles as distinct structures with well supported independent origins in bugs and beetles to investigate the nature of molecular convergence in ejaculate structures using comparative proteomics. Results from comparisons with previously characterized whirligig beetle (Gyrinidae) proteomes reveals true bugs have converged in their use of sperm leucyl aminopeptidases (S-LAPs) to construct spermatostyles. The SLAP protein families are found in high abundance in all speramtostyle proteomes excluding the cicada, and the independent recruitment of S-LAPs in both taxa may represent evidence of a common underlying toolkit available to insects when structuring their ejaculates.
2026-04-29 | PXD064531 | Pride
Project description:In this study we applied RNA-sequencing to a set of larvae-challenged elm trees that had either been untreated before or been exposed to prior egg deposition by elm leaf beetles. This dataset allowed us to characterize the global transcriptional response of egg-primed and non-primed elm trees at different time points after the priming stimulus itself and after the onset of larval feeding.
2018-10-04 | GSE77985 | GEO
Project description:Firefly genomes illuminate parallel origins of bioluminescence in beetles
| PRJNA419213 | ENA
Project description:Gene expression estimates detected by RNA-sequencing technology vary with the updates of reference genome and gene annotation, which might confound existing expression-based prognostic signatures, making them inapplicable to clinical practice. In this study, we proposed a method to decrease these effects and developed a qualitative signature for stage I lung adenocarcinoma, whose classification was based on within-sample relative expression orderings (REOs) of gene pairs. The signature was validated in 471 stage I samples derived from public RNA-sequencing and microarray data (both log-rank p < 0.001). Notably, our signature could effectively predict prognosis for 30 stage I patients with severely degraded FFPE tissues (log-rank p = 0.0177). More important, the risk classification was stable in the latest annotation. In summary, our signature would be a promising signature for clinical individualized application because of its excellent prognostic performance and classification robustness.
2020-01-13 | GSE143486 | GEO
Project description:Fireflies and their luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin of bioluminescence within beetles remains unclear. To shed light on this long-standing question, we sequenced the genomes of two firefly species that diverged over 100 million-years-ago: the North American Photinus pyralis and Japanese Aquatica lateralis. To compare bioluminescent origins, we also sequenced the genome of a related click beetle, the Caribbean Ignelater luminosus, with bioluminescent biochemistry near-identical to fireflies, but anatomically unique light organs, suggesting the intriguing hypothesis of parallel gains of bioluminescence. Our analyses support independent gains of bioluminescence in fireflies and click beetles, and provide new insights into the genes, chemical defenses, and symbionts that evolved alongside their luminous lifestyle.
2018-09-14 | MTBLS698 | MetaboLights