Project description:We compiled the transcriptome by extracting mRNA, reverse transcription and Illumina sequencing, followed by assembly and annotation by comparison with public databases (including; Nr, SwissProt and COG). Quantitative data on the transcription abundance of each putative protein sequence is provided as assesed by the SOAPdenovo_trans assembly tool.
Project description:Global transcriptome analysis of early and terminal erythropoiesis in newly diagnosed with multiple myeloma patients associated anemia
Project description:We developed a method to deconvolute decays of m6A mRNAs and their unmethylated isoforms from newly synthesized total RNAs across the transcriptome to study the effects of m6A modification on decays of not only the m6A-modified RNA pool but also the unmehnylated isoform pool.
Project description:Using a newly-developed workflow for quantitative newly synthesized proteome analysis (QuaNPA), featuring automated sample processing and multiplexed DIA (plexDIA) analysis, changes in the newly synthesized proteome of IFN-gamma treated Hela cells were monitored over time.