Project description:We used RNA-seq to examine transcriptional proflies of midguts with suppression of Imd in progenitors (e_* samples) or enterocytes (M_* samples) . We found significant differences between the contributions of enterocyte IMD and progenitor IMD to intestinal homeostasis.
Project description:The inner membrane domain (IMD) is a metabolically active and laterally discrete membrane domain initially discovered in Mycobacterium smegmatis that correlates both temporally and spatially with polar cell envelope elongation. While it has remained unclear whether a similar membrane domain exists in pathogenic species, this study specifically demonstrates that the IMD is a conserved membrane structure found in Mycobacterium tuberculosis. Following isolation of the membrane domains by density gradient fractionation, proteomic analysis revealed that the IMD is enriched in metabolic enzymes that are involved in the synthesis of conserved cell envelope components such as peptidoglycan, arabinogalactan, and phosphatidylinositol mannosides. Further, by demonstrating that the IMD is concentrated in the polar region of the rod-shaped cells, where active cell envelope biosynthesis takes place, proteomic analysis further revealed the enrichment of enzymes involved in synthesis of phthiocerol dimycocerosates and phenolic glycolipids. Overall, these proteomic data support that the functional compartmentalization of the membrane is an evolutionarily conserved feature found in both M. tuberculosis and M. smegmatis.
Project description:The purpose of this RNAseq is to analyse the effect of compound IMD-0354 on gene expression in melanoma A375 cells. RNAseq analysis identified unfolded protein response, cell cycle and DNA damage pathways to be effected by IMD-0354.
Project description:We used RNA-seq to examine transcriptional profiles of midgut progenitor cells with suppression of Imd in progenitors. We found significant differences between the contributions of progenitor IMD to intestinal homeostasis.
Project description:Activation of innate immune responses in the Drosophila larval fat body affects the physiological host responses. In order to characterize the effect of the activated immune responses in the fat body on the Drosophila, we used whole-genome microarray analysis and found that activation of the immune deficieny pathway (Imd) in the fat body alters the transcriptional profiles of Drosophila larvae. As we expected many of genes involved in regulation of antimicrobial peptides were upregulated in the larvae with elevated Imd activity in the fat body. Notably, we found activatioan of Imd in the fat body negatively affects expression of genes involved in glycolysis, energy production and insulin signaling pathway. Overall, our analysis showed that activation of innate immune signaling in the larval fat body significantly affects cellular pathways that regulate metabolism.