Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
Project description:This dataset was utilized to assess the performance of a novel de novo metaproteomics pipeline, which performs sequence alignment of de novo sequences from complete metaproteomics experiments. Traditionally, metaproteomics data annotation relies on database searching that requires sample-specific databases derived from whole metagenome sequencing experiments. Creating these databases, however, is a complex, time-consuming, and error prone process, which can introduce biases affecting the outcomes and conclusions, highlighting the need for alternative methods. The evaluated approach offers rapid and orthogonal insights into metaproteomics data.