Project description:This data set contains bacteria isolated from corals. The bacteria were cultured and tested in a biological assay against pathogenic bacteria that may be involved in Stony Coral Tissue Loss Disease. The bacteria in this data set include pathogenic and potentially probiotic bacteria.
Project description:Isolation of bacteria in infected brains in patients with Parkinson's disease. Here we used next generation sequencing of 16S ribosomal RNA gene PCR amplicons (NGS 16S amplicon analysis).
Project description:Purpose: Examining the transcriptome of human gut bacteria that grow on seaweed polysaccharides as a sole carbon source Methods: Strains were grown on 5 mg/ml seaweed polysaccharides (carrageenan, agarose and/or poprhyran respective to strain) or galactose as a sole carbon source in vitro. Fold change was calculated as seaweed polysaccharide over galactose with n=2 biological replicates. Once cells reached an optical density corresponding to mid-log phase growth, RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >10-fold and with both biological replicates with a normalized expression level >1% of the overall average RPKM expression level. READS WERE ANALYZED .......GABRIEL FILL IN Results: We identified novel polysaccharide utiilization loci in 5 strains of human gut bacteria
Project description:Whole genome sequence resources for Pseudomonas amygdali pv. sesami and Xanthomonas arboricola isolated from sesame (Sesamum indicum) in North Carolina in 2022
Project description:This data set contains bacteria isolated from corals. The bacteria were cultured and tested in a biological assay against pathogenic bacteria that may be involved in Stony Coral Tissue Loss Disease. The bacteria in this data set include pathogenic and potentially probiotic bacteria.
Project description:Bacterial Isolate Assemblies for Microbiota Restoration Reduces Antibiotic Resistant Bacteria Gut Colonization in Patients with Recurrent Clostridioides difficile Infection from the Open-Label PUNCH CD Study, Nov 01 '20
Project description:Multipartite bacterial genome organization can confer advantages including coordinated gene regulation and faster genome replication, but is challenging to maintain. Agrobacterium tumefaciens lineages often contain a circular chromosome (Ch1), a linear chromosome (Ch2), and multiple plasmids. We previously observed that in some stocks of the lab model strain C58, Ch1 and Ch2 were fused into a linear dicentric chromosome. Here we analyzed Agrobacterium natural isolates from the French Collection for Plant-Associated Bacteria (CFBP) and identified two strains with fused chromosomes. Chromosome conformation capture identified integration junctions that were different from the C58 fusion strain. Genome-wide DNA replication profiling showed both replication origins remained active. Transposon sequencing revealed that partitioning systems of both chromosome centromeres were essential. Importantly, the site-specific recombinases XerCD are required for the survival of the strains containing the fusion chromosome. Our findings show that replicon fusion occurs in natural environments and that balanced replication arm sizes and proper resolution systems enable the survival of such strains.