Project description:To elucidate the molecular mechanisms of tumor growth inhibition caused by the anti-LN-332 antibodies, we plated human A431 cells on plates coated with fibroblast-derived matrix for 6 hours and then added a mixture of antibodies against all three LN-332 chains or control IgG to the medium for 16 hours. All cells from antiobody treated and controls were collected and subjected to global transcriptome analysis using Affymetrix Human Genome U133 Plus 2.0 Arrays.
Project description:Oryza sativa Japonica Group CM, Similar to Chorismate mutase, chloroplast precursor (EC 5.4.99.5) (CM-1), is differentially expressed in 29 experiment(s);
Project description:Oryza sativa Japonica Group CM, Similar to Chorismate mutase, chloroplast precursor (EC 5.4.99.5) (CM-1), is expressed in 14 baseline experiment(s);
Project description:Saliva based diagnostics is a rapidly evolving field due to the large potential of saliva and the simple sample collection. A systematic comparison of IgG antibody profiles in saliva and plasma is currently lacking in scientific literature. Our hypothesis is that IgG profiles are equal in blood and saliva. By showing the equality of the profiles and relative IgG antigenic reactivities towards proteins and peptides we provide evidence that plasma IgG reactivities can be inferred from saliva IgG reactivities. IgG antibodies were isolated from human saliva and plasma samples. The reactivities of IgG isolates were analysed on peptide microarrays displaying linear epitopes of EBV (EBNA1 protein) and HBV (Large envelope protein) virus. Peptide arrays were printed by JPT Peptide Technologies (Berlin, Germany). We show high similarity of saliva and plasma IgG profiles on these two platforms and argue for generalisation from this subset to the whole immunological IgG antibody profile.
