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Bacterial community composition in phyllosphere and rhizosphere of woolly beech aphid infestated beech

ABSTRACT: Bacterial community composition in the phyllosphere and rhizosphere of beech (Fagus sylvatica), subjected to woolly beech aphid (Phyllaphis fagi) infestation in a mesocosm experiment

PROVIDER: PRJEB46752 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR6397250_1.fastq.gz	Fastqsanger.gz
	ERR6397250_2.fastq.gz	Fastqsanger.gz
	ERR6397260_1.fastq.gz	Fastqsanger.gz
	ERR6397260_2.fastq.gz	Fastqsanger.gz
	ERR6397241_1.fastq.gz	Fastqsanger.gz

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Project description:Young Fagus sylvatica trees (approximately 7 to 8 years) were collected from a natural regeneration beech forest. The trees were excavated with intact soil cores, roots and top organic layer. The trees were then kept outdoors at the Department of Forest Botany, Georg-August-Universität Göttingen. Plants were protected from rain, and light conditions were matched to those of the natural stand using a shading net; otherwise, plants were exposed to natural climatic conditions. The soil moisture was regularly measured; plants were watered with deionized water as needed to keep soil moisture close to the original conditions. Trees was randomly relocated on a weekly basis throughout the experiment to avoid biasses caused by location or light effects. After 21 weeks, a treatment was applied to understand the physiological mechanisms of inorganic nitrogen uptake and assimilation under conditions of an inorganic nitrogen saturated forest simulation: Plants were fertilized with either a 20 mM solution of KNO3, a 20 mM solution of NH4Cl, or demineralized water (control) for 2 days. On the third day, the trees were harvested. Root tips were immediately shock-frozen in liquid nitrogen and used for RNA extraction.

2020-12-14 | E-MTAB-8931 | biostudies-arrayexpress

Project description:Phyllaphis fagi overview

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