Project description:This study aimed at identification of genetic regulations for desiccation tolerance in intertidal seaweed species Ulva lactuca most commonly experienced phenomenon of intertidal communities.
Project description:This study aimed at identification of genetic regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca which arisies periodically due to tidal rhythms.
Project description:Exploiting the full potential of insertional mutagenesis screens with retroviruses and transposons requires methods for distinguishing clonal from subclonal insertion events within heterogeneous tumor cell populations. Current protocols, based on ligation mediated PCR, depend on endonuclease based fragmentation of genomic DNA, resulting in strong biases in amplification and sequencing due to a fixed product sizes of the amplicon. We have developed a method called shear-splink, which enables the semi-quantitative high-throughput sequence analysis of insertional mutations, enabling us to count the number of cells harboring a given integration, within a heterogeneous sample. The shear-splink method enriches for (sub)clonal integrations, thereby reducing the contribution of irrelevant passenger mutations normally hampering a reliable identification of common integration sites. Additionally, this improvement allows us to identify genetic interactions between affected genes, co-occurring mutations and to study acquired resistance mechanisms both in vivo and in vitro.
Project description:Ulva sp., a promising yet underexplored food source, offers rich protein content and nutritional benefits. However, assessing its allergenicity remains challenging due to limited research and understanding. In this translational research, we focus on a laboratory-produced Ulva sp. product (UlvaLe) and gather comprehensive data to assess its potential allergenicity. Therefore, we employed a weight-of-evidence approach inspired by regulatory standards, conducting a literature review, proteomic analysis, and clinical trial. This article addresses challenges in assessing the allergenicity of novel foods, using Ulva sp. as an example, while enhancing the understanding of Ulva sp.'s allergenic potential and offers insights for future research and regulatory processes. Our findings reveal no reported cases of Ulva allergenicity in the literature but highlight the need to address polysaccharide safety. Proteins with homology exceeding 70% to known allergens were identified as putative allergens. Nonetheless, interim clinical trial results demonstrate no allergic reactions to UlvaLe ingestion, suggesting a favorable safety profile. The presented data suggests a promising allergenicity safety profile for UlvaLe as a novel food source. Further research into macro-algae allergenicity, including DNA and protein composition decoding, is warranted to ensure safe utilization in the food industry. Nevertheless, the most effective means of assessing allergenicity for any new food is through widespread consumption among large populations, which statistically reveals the true public risk. As a modern society seeking innovative solutions and sustainable growth, it is imperative to be mindful of this and devise strategies for its safe integration.
