Project description:We used Nimblegen arrays with design 2007-11-06_Smed_ESTs_4_exp to measure changes in gene expression during a timecourse of regeneration of one side of the head in Schmidtea mediterranea flatworms. Following amputation of one side of the head, samples were collected from both the regeneration blastema and non-regenerating side of the head on days 2, 3, and 4 post-amputation. Gene expression in these samples was compared to that in non-regenerating control samples collected at the time of amputation.
Project description:We report the expression of mRNA transcripts in X1 neoblast isolated by FACS followin amputation of the head (anterior wounding) and amputation of the pharyx and tail (posterior wounding) at 0 and 48 hours
Project description:We report the expression of mRNA transcripts in X1 neoblast isolated by FACS followin amputation of the head (anterior wounding) and amputation of the pharyx and tail (posterior wounding) at 0 and 48 hours RNA-sequencing of pre-pharyngeal X1 cells from anterior and posterior facing wounds at 0 and 48 hrs after wounding in S. mediterranea
Project description:X1 neoblasts (G2/S/M cells) were isolated by FACS from injury site-proximal tissue in a timeseries during head (anterior) and tail (posterior) regeneration in Schmidtea mediterranea 24 samples: 2 treatments (head versus tail regeneration), 3 timepoints (0,24,48,72 hours post amputation), 3 biological replicates each condition
Project description:Sexual Schmidtea mediterranea Trunk Regeneration. Sexual biotype Schmidtea mediterranea trunk fragments were sequenced at 0 day, 3 day, 5 day and 7 day post amputation along with whole worm juvenile animals in order identify sexual specific transcripts. Juvenile worms lack sex organs, testes and ovaries present in D0 trunk fragments. Days 3-7 represent time points of degeneration and regeneration of the sexual organs, testes and ovaries.
Project description:RNA sequencing identified new anterior-pole enriched genes encoding transcription factors, a secreted protein, and a transmembrane protein, including NR4A, a nuclear receptor gene that is important in patterning the planarian head and tail.
Project description:RNA sequencing identified various nr4A-regulated genes in the head and tail, including a large number of muscle-enriched genes and many positional control genes, most of which were down-regulated by nr4A inhibition.
