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Genomic analysis of Burkholderia pseudomallei

ABSTRACT: Burkholderia pseudomallei causing mellidiosis is more common in INDIA

PROVIDER: PRJEB47192 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR6565140_1.fastq.gz	Fastqsanger.gz
	ERR6565140_2.fastq.gz	Fastqsanger.gz
	ERR6565150_1.fastq.gz	Fastqsanger.gz
	ERR6565150_2.fastq.gz	Fastqsanger.gz
	ERR6565160_1.fastq.gz	Fastqsanger.gz

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Genomic Loss of Penicillin-Binding Protein 3 in Burkholderia pseudomallei Confers Clinical Resistance to Ceftazidime

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Role of ppiB in burkholderia pseudomallei

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Project description:Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is a disease endemic to South-East Asia and Northern Australia. Clinical presentation is highly variable, ranging from asymptomatic to fatal septicaemia, and thus the outcome of infection can depend on the host immune responses. The aim of this study was to characterise the macrophage immune response to B. pseudomallei in the presence of novel inhibitors targeting the virulence factor, Macrophage Infectivity Potentiator (Mip) protein. To do this. murine macrophage J774A.1 cells were infected with B. pseudomallei K96243 in the presence and absence of two small-molecule inhibitors designed to target the Mip protein. Global transcriptional profiling of macrophages infected with B. pseudomallei was analysed by RNA-Seq four hours post-infection. In the presence of Mip inhibitors, we found a significant reduction in the expression of pro-inflammatory cytokines highlighting the potential to utilize Mip inhibitors to dampen potentially harmful pro-inflammatory responses resulting from B. pseudomallei infection in macrophages. We then performed gene expression profiling analysis using data obtained from RNA-seq of J774A.1 macrophages infected with Burkholderia pseudomallei in the presence of two Mip inhibitors or vehicle control 4 hours post-infection

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The expression of virulence and antimicrobial related proteins in different Burkholderia mallei and Burkholderia pseudomallei isolates

Project description:Burkholderia mallei and Burkholderia pseudomallei are both potential biological threats agents. Melioidosis caused by B. pseudomallei is endemic in Southeast Asia and Northern Australia, while glanders caused by B. mallei infections are rare. Here we studied the proteomes of different B. mallei and B. pseudomallei isolates to determine species specific characteristics. Analyzing the expressed proteomes of B. mallei and B. pseudomallei revealed differences between B. mallei and B. pseudomallei but also between isolates from the same species. Expression of multiple virulence factors and proteins of several PKS/NRPS clusters was demonstrated. Proteome analysis can be used not only to identify bacteria but also to characterize the expression of important factors that putatively contribute to pathogenesis of B. mallei and B. pseudomallei.

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