Project description:Investigation of global gene expression changes in Saccharomyces cerevisiae strain NRRL Y-12632 (ATCC® 18824) grown in media made with asbestos mine tailings-laden water compared to the control grown in media made with double distilled water
Project description:Bacterial communities from three different sampling sites of a copper mine tunnel were characterized by 16S rRNA sequencing (NGS). A high presence of halophilic bacteria was confirmed by comparison with literature data and with reference samples from other highly salt-exposed soils. Among others, high read numbers of Gracilimonas, Kangiella, Limibacillus, Marinobacter, Woseia, and uncultivated strains of Actinomarinales, Gammaproteobacterium AT-s16, Actinobacteria 0319-7L14, and Thiotrichaceae were found. The community in a sample from the surface of the copper seam was significantly different from the community composition of a sample from the mine tunnel floor. The specificity in the appearance and in the abundance of special bacterial types (for example, Thiogranum, Thiohalophilus, Sulfuriflexus, Sedimenticolaceae, Desulfomonile, Desulfosporosinus, and Cand. Thiobios) can be partially explained by the different local conditions for sulfur-related metabolisms at the sampling sites.
Project description:The pyrite mine of Lousal, Grândola, Portugal, was discovered in 1882 and extensively exploited from 1900 until its closure in 1988 when the mining ores were not viable economically. Aiming at becoming a cultural-touristic center, the mining area was rehabilitated raising a Mining Museum and a Science Centre along with the reopening of a mining gallery for public access. In recent times, aerobiological studies on subterranean environments such as caves, mines and archeological necropolis revealed to be an important source of novel bacteria, from which their biotechnological potential are under study. Here we present a Pseudomonas sp. isolated from the mine air with potential secondary metabolite biosynthesis, resistance to antibiotics and virulence factors.
