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Msn2-mediated nucleosome remodeling dynamics

ABSTRACT: Msn2-mediated nucleosome remodeling dynamics

PROVIDER: PRJEB4737 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR351462_1.fastq.gz	Fastqsanger.gz
	ERR351462_2.fastq.gz	Fastqsanger.gz
	ERR351463_1.fastq.gz	Fastqsanger.gz
	ERR351463_2.fastq.gz	Fastqsanger.gz
	ERR351464_1.fastq.gz	Fastqsanger.gz

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Msn2-mediated nucleosome remodeling dynamics

Project description:The aim of this experiment was to determine, using MNAse-Seq, how nucleosomes get remodeled during an Msn2 activation timecourse genome-wide. Diploid strain EY2807/ASH79 with genotype TPK1M164G TPK2M147G TPK3M165G msn4::TRP1/LEU2 MSN2-mCherry NHP6a-iRFP::kanMX was used. This strain is PKAas, so upon addition of the inhibitor 1-NM-PP1, Msn2 translocates to the nucleus and activates gene expression. In this experiment, the diploid strain was exposed to 3 uM 1-NM-PP1 for 0, 5, 10, 20 and 40 min and nucleosome positions determined using by crosslinking, MNAse treatment, nucleosomal DNA purification and paired-end high-throughput sequencing (Illumina). Results from transcriptional profiling of yeast with or without Msn2 expression were also deposited at ArrayExpress under accession number E-MTAB-1945 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1945/ ).

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Stochastic models of nucleosome dynamics reveal regulatory rules of stimulus-induced epigenome remodeling

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Project description:This SuperSeries is composed of the following subset Series: GSE30897: Nucleosome occupancy in yeast BY4741and a strain lacking MSN2 and MSN4 responding to 20 min treatment with 0.4mM H2O2 GSE30898: Msn2p occupancy dynamics in yeast BY4741 responding to 0.4mM H2O2 over time (0-60 min) GSE30899: Gene expression dynamics in yeast BY4741 and a strain lacking MSN2 and MSN4 responding to 0.4mM H2O2 over time (0-60min) GSE30900: Nucleosome occupancy dynamics in yeast BY4741 responding to 0.4mM H2O2 over time (0-60 min) Refer to individual Series

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