Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

P-element in Drosophila simulans at cold conditions

ABSTRACT: P-element invasions at cold conditions in experimentally evolving Drosophila simulans populations

PROVIDER: PRJEB47624 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR6797480_1.fastq.gz	Fastqsanger.gz
	ERR6797480_2.fastq.gz	Fastqsanger.gz
	ERR6797490.fastq.gz	Fastqsanger.gz
	ERR6797471_1.fastq.gz	Fastqsanger.gz
	ERR6797471_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 45

Similar Datasets

Drosophila simulans

Project description:Drosophila simulans P-element sequences

| PRJNA312016 | ENA

Drosophila simulans

Project description:Drosophila simulans targeted loci P-element

| PRJNA312047 | ENA

Project description:P-element invasions in D. melanogaster and D. simulans

| PRJNA997230 | ENA

D. simulans with variable Y chromosomes

Project description:Y chromosome from different Drosophila simulans were introgressed into the same genetic background. Strains showing distinct sex-ratio distortion were clustered according to the ratio of males and females observed in the progeny. Strains showing disproportionally high number of female flies in the progeny were contrasted with strains displaying the proportion of male and females flies close to 1:1. In addition, interspecific Y chromosome from Drosophila sechellia (Ysec) was compared with D. simulans Y chromosomes. Total RNA was extracted from whole flies using TRIzol (Life Technologies). The synthesis of cDNA and its labeling with fluorescent dyes (Cy3 and Cy5) as well as hybridization reactions were carried out using 3DNA protocols and reagents (Genisphere). A minimum of 2 biological replicas was used, however this number varied according to the hybridization design (See 'Branco et. al, Natural variation of the Y chromosome suppresses sex ratio distortion and modulates testis-specific gene expression in Drosophila simulans' for details of the hybridization design). Microarrays were ~18,000-feature cDNA arrays spotted with D. melanogaster cDNA PCR products. We have recently annotated these probes using the D. simulans genome. Possible biases introduced by using a D. melanogaster platform for a D. simulans genome are minimized because the probed genomes are essentially identical except for the Y chromosomes. Slides were scanned using Axon 400B scanner (Axon Instruments) and GenePix Pro 6.0 software. Foreground fluorescence of dye intensities was normalized by the Loess method in Bioconductor / Limma.

2014-02-01 | GSE43665 | GEO

Drosophila simulans blue gut larvae vs. white prepupae

Project description:Drosophila simulans blue gut larvae vs. white prepupae Keywords: repeat sample

2003-01-27 | GSE134 | GEO

Gene expression analysis of interspecific and intraspecific Y chromosome introgessions in Drosophila simulans

Project description:These arrays measure gene expression across eight Y introgression lines in Drosophila simulans. Four lines (Ya19, Ya23, Ya24, Ya26) carry a D. simulans Y chromosome (from a Cameroon population) and four lines (Sec01, Sec03, Sec08, Sec27) carry a D. sechellia Y chromosome. All lines are otherwise identical with a D. simulans background (UCSD stock center line 14021-0251.092).

2011-09-30 | GSE31907 | GEO

D. simulans with variable Y chromosomes

2014-02-01 | E-GEOD-43665 | biostudies-arrayexpress

Ricania simulans

Project description:Ricania simulans overview

| PRJNA1047249 | ENA

Staphylococcus simulans

Project description:Staphylococcus simulans overview

| PRJNA52247 | ENA

Sargassococcus simulans

Project description:Sargassococcus simulans Transcriptome

| PRJNA732377 | ENA