Project description:To identify oilseed rape genes with a potential role in N-remobilization during leaf senescence of developmentally old leaves in the lower canopy and young leaves in the upper canopy, transcriptomes of leaf number 4 and leaf number 8 of B. napus (cultivar Mozart) were analysed at different harvest time points under mild N deficiency and optimal N fertilization.
2018-02-01 | GSE97653 | GEO
Project description:Trees microbiome from tropical reservoir
| PRJNA941258 | ENA
Project description:Bacterial vertical distribution during thermal stratification of drinking reservoir
Project description:The host and microbiome coexist in the natural environment, engaging in constant and dynamic interactions that are fundamental to human health. Understanding these interactions requires unbiased, spatially resolved techniques capable of mapping gene expression in both the host and microbiome within their native microenvironment. In this study, we introduce microDBiT, a spatial sequencing technology that enables simultaneous, high-resolution profiling of both host and bacterial RNAs (mRNA and rRNA). By applying microDBiT, we successfully mapped the spatial distribution of host and bacterial RNAs in the intestines, colon, and tongue of germ-free (GF) mice colonized with bacteria. Furthermore, we utilized this approach in a dextran sulfate sodium (DSS)-induced colitis model, uncovering key mRNA-level changes in both host and microbial cells. This study demonstrates the power of microDBiT in resolving host-microbiome interactions at the transcriptomic level, providing critical insights into host-pathogen dynamics, immune responses, and microbiome-driven disease mechanisms.
Project description:The gut microbiome is a malleable microbial community that can remodel in response to various factors, including diet, and contribute to the development of several chronic diseases, including atherosclerosis. We devised an in vitro screening protocol of the mouse gut microbiome to discover molecules that can selectively modify bacterial growth. This approach was used to identify cyclic D,L-α-peptides that remodeled the Western diet (WD) gut microbiome toward the low-fat-diet microbiome state. Daily oral administration of the peptides in WD-fed LDLr-/- mice reduced plasma total cholesterol levels and atherosclerotic plaques. Depletion of the microbiome with antibiotics abrogated these effects. Peptide treatment reprogrammed the microbiome transcriptome, suppressed the production of pro-inflammatory cytokines (including interleukin-6, tumor necrosis factor-α and interleukin-1β), rebalanced levels of short-chain fatty acids and bile acids, improved gut barrier integrity and increased intestinal T regulatory cells. Directed chemical manipulation provides an additional tool for deciphering the chemical biology of the gut microbiome and might advance microbiome-targeted therapeutics.
2019-12-31 | GSE104913 | GEO
Project description:Bacterial community analysis of 100 tropical rainforest trees
| PRJNA972553 | ENA
Project description:Mutation in the tropical tree canopy
Project description:This dataset comprises raw LC-MS data generated from a study examining the root and rhizosphere metabolomes of of Lehmann lovegrass (Eragrostis lehmanniana) and Arizona cottontop (Digitaria californica) in both canopy and inter-canopy regions under velvet mesquite trees (Prosopis velutina) at the Santa Rita Experimental Range (SRER) in Arizona. Samples were collected from twelve 1m x 1m plots representing two dominant plant species (Lehmann lovegrass and Arizona cottontop) across two canopy conditions (closed and open), resulting in 36 root and rhizosphere samples.