Project description:The some biomarkers can be found by pairwise comparison. They can distinguish between extremely severe Hand,foot and mouth disease and mild Hand,foot and mouth disease,moreover,they can applied to diagnose extremely severe Hand,foot and mouth disease mild Hand,foot and mouth disease vs.control; extremely severe Hand,foot and mouth disease vs.control; extremely severe Hand,foot and mouth disease vs.mild Hand,foot and mouth disease,verification by qRT-PCR
Project description:The some biomarkers can be found by pairwise comparison. They can distinguish between extremely severe Hand,foot and mouth disease and mild Hand,foot and mouth disease,moreover,they can applied to diagnose extremely severe Hand,foot and mouth disease
Project description:To elucidate alterations in immune cells during enterovirus 71 (EV-A71) infection and explore potential interaction mechanisms.Single-cell sequencing technology was used to sequence peripheral blood monocytes (PBMCs) obtained from a severe hand, foot and mouth disease (HFMD) patient due to EV-A71 and a healthy control.
Project description:Abstract: Hand, foot, and mouth disease (HFMD) is a widespread viral infection predominantly affecting young children, for which specific therapies remain elusive despite extensive research efforts. N6-methyladenosine (m6A) modifications, prevalent in eukaryotic mRNA and long non-coding RNAs, are also found in various viral RNAs. A nuclear methyltransferase complex, including METTL3 as the catalytic subunit, catalyzes these m6A modifications co-transcriptionally, functioning as m6A 'writers'. Notably, enterovirus 71 (EV71) RNA undergoes m6A modification during infection, mediated by METTL3, influencing virus replication. We hypothesized that inhibiting METTL3 could directly suppress EV71 replication through m6A modifications. Despite the renown of STM2457 as a METTL3 inhibitor, our preliminary experiments indicated its ineffectiveness against EV71. Leveraging a compound library tenfold larger than that in STM2457's discovery, we identified AN465 as a potent METTL3 inhibitor. Through rigorous experiments and analyses, we demonstrate that AN465 surpasses STM2457 in inhibiting EV71 replication by effectively targeting METTL3. Our findings propose AN465 as a promising therapeutic candidate for combating HFMD by curbing EV71 replication.
Project description:We report a large-scale outbreak of hand, foot and mouth disease (HFMD) in France. As at 28 September 2021, 3,403 cases have been reported (47% higher than in 2018-19). We prospectively analysed 210 clinical samples; 190 (90.5%) were enterovirus-positive. Most children presented with atypical HFMD. Coxsackievirus (CV)A6 (49.5%; 94/190) was predominant; no enterovirus A71 was detected. Dermatological and neurological complications of HFMD justify prospective syndromic and virological surveillance for early detection of HFMD outbreaks and identification of associated types.
Project description:The combinational use of probiotics and prebiotics supplement lower the infection risk of hand-foot-and-mouth disease in children potentially by providing resistance to the gut microbiota dysbiosis