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Introgression of a synthetic sex ratio distortion into Anopheles coluzzii

ABSTRACT: Introgression of a synthetic sex ratio distortion transgene into local genetic backgrounds of Anopheles coluzzii

PROVIDER: PRJEB48699 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR7457690_1.fastq.gz	Fastqsanger.gz
	ERR7457690_2.fastq.gz	Fastqsanger.gz
	ERR7457691_1.fastq.gz	Fastqsanger.gz
	ERR7457691_2.fastq.gz	Fastqsanger.gz
	ERR7457692_1.fastq.gz	Fastqsanger.gz

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Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii.

Project description:The development of genetically modified mosquitoes (GMM) and their subsequent field release offers innovative approaches for vector control of malaria. A non-gene drive self-limiting male-bias Ag(PMB)1 strain has been developed in a 47-year-old laboratory G3 strain of Anopheles gambiae s.l. When Ag(PMB)1 males are crossed to wild-type females, expression of the endonuclease I-PpoI during spermatogenesis causes the meiotic cleavage of the X chromosome in sperm cells, leading to fertile offspring with a 95% male bias. However, World Health Organization states that the functionality of the transgene could differ when inserted in different genetic backgrounds of Anopheles coluzzii which is currently a predominant species in several West-African countries and thus a likely recipient for a potential release of self-limiting GMMs. In this study, we introgressed the transgene from the donor Ag(PMB)1 by six serial backcrosses into two recipient colonies of An. coluzzii that had been isolated in Mali and Burkina Faso. Scans of informative Single Nucleotide Polymorphism (SNP) markers and whole-genome sequencing analysis revealed a nearly complete introgression of chromosomes 3 and X, but a remarkable genomic divergence in a large region of chromosome 2 between the later backcrossed (BC6) transgenic offspring and the recipient paternal strains. These findings suggested to extend the backcrossing breeding strategy beyond BC6 generation and increasing the introgression efficiency of critical regions that have ecological and epidemiological implications through the targeted selection of specific markers. Disregarding differential introgression efficiency, we concluded that the phenotype of the sex ratio distorter is stable in the BC6 introgressed An. coluzzii strains.

| S-EPMC10092091 | biostudies-literature

Transmission ratio distortion is frequent in Arabidopsis thaliana controlled crosses

Project description:We have performed a species-wide screen for transmission ratio distortion in over 500 segregating F2 populations of Arabidopsis thaliana using pooled reduced-representation genome sequencing.

| PRJEB27214 | ENA

Anopheles coluzzii

Project description:Anopheles coluzzii overview

| PRJNA300899 | ENA

Transmission ratio distortion results in asymmetric introgression in Louisiana Iris.

Project description:BackgroundLinkage maps are useful tools for examining both the genetic architecture of quantitative traits and the evolution of reproductive incompatibilities. We describe the generation of two genetic maps using reciprocal interspecific backcross 1 (BC1) mapping populations from crosses between Iris brevicaulis and Iris fulva. These maps were constructed using expressed sequence tag (EST)- derived codominant microsatellite markers. Such a codominant marker system allowed for the ability to link the two reciprocal maps, and compare patterns of transmission ratio distortion observed between the two.ResultsLinkage mapping resulted in markers that coalesced into 21 linkage groups for each of the reciprocal backcross maps, presumably corresponding to the 21 haploid chromosomes of I. brevicaulis and I. fulva. The composite map was 1190.0-cM long, spanned 81% of the I. brevicaulis and I. fulva genomes, and had a mean density of 4.5 cM per locus. Transmission ratio distortion (TRD) was observed in 138 (48.5%) loci distributed in 19 of the 21 LGs in BCIB, BCIF, or both BC1 mapping populations. Of the distorted markers identified, I. fulva alleles were detected at consistently higher-than-expected frequencies in both mapping populations.ConclusionsThe observation that I. fulva alleles are overrepresented in both mapping populations suggests that I. fulva alleles are favored to introgress into I. brevicaulis genetic backgrounds, while I. brevicaulis alleles would tend to be prevented from introgressing into I. fulva. These data are consistent with the previously observed patterns of introgression in natural hybrid zones, where I. fulva alleles have been consistently shown to introgress across species boundaries.

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Anopheles coluzzii RNAseq

Project description:Anopheles coluzzii RNAseq

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Anopheles coluzzii

Project description:Anopheles coluzzii (African malaria mosquito)

| PRJNA648422 | ENA

Diospyros lotus

Project description:Persimmon sex ratio distortion

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Anopheles coluzzii

Project description:Anopheles coluzzii principal pseudohaplotype genome sequencing