Project description:Background: Chronic obstructive pulmonary disease (COPD) is a serious chronic disease of the airways that affects many people worldwide and have limited treatment options. While small animal models provide a platform for therapeutic investigations into COPD, their deficiencies continue to impede clinical translation. Alternatively, as a large animal model, sheep have a respiratory system anatomically and physiologically similar to that of humans, encouraging their use in airway disease research. The aim of this study was to better understand disease pathology in a large animal (sheep) experimental model of COPD. Methods: COPD was induced in sheep following lung exposure to porcine elastase (PE) and repeated weekly lung exposures to lipopolysaccharide (LPS) over a period of 8 weeks. Bronchoalveolar fluid and blood samples were collected for immune analyses. Lung function was assessed and lung tissues were collected for histopathology and RNA sequencing. Results: Lung neutrophil levels were elevated in response to repeated airway exposure to PE/LPS, accompanied by a significant decline in ventilation over time. Histological evidence of COPD-like disease changes included chronic inflammation with increased airway and tissue inflammation scores, together with significantly larger airway wall area measures, increased connective tissue deposition and dysregulated gene expression. Conclusions: These studies demonstrate sustained chronic airway inflammation and pathophysiological lung changes in a sheep model of COPD, providing many similarities to that seen in COPD patients. This work opens a pathway for future translational studies using this unique large animal model of COPD, which will serve to bridge the gap between smaller animal models and humans.

2023-12-31 | GSE242416 | GEO

PNET animal model: new insights

Project description:Recently, we described a new animal model of CNS primitive neuroectodermal tumors (CNS-PNET), which was generated by orthotopic transplantation of human Radial Glial (RG) cells into NOD-SCID mice’s brain sub- ventricular zone. In the current study we conducted comprehensive RNA-Seq analyses to gain some insights on the mechanisms underlying tumorigenesis in this mouse model of CNS-PNET. Here we show that the RNA-Seq profiles derived from these tumors cluster with those reported for patients’ PNETs.

2017-06-12 | GSE82102 | GEO

Forced intensity-controlled endurance training on a small-animal treadmill machine inducing murine cardiac hypertrophy

Project description:Endurance training is associated with decreased cardiovascular-related morbidity and mortality. Cardiac hypertrophy is an adaptive mechanism of the heart in response to volume and pressure overload. Nonetheless, murine exercise models for cardiac hypertrophy are still under discussion. Using a small-animal treadmill, a forced intensity controlled training model was conducted to characterize cardiac hypertrophy in mice utilizing multimodal analyses. Wild-type male C57BL/6 mice age between 5-8 weeks old were subjected to 8 weeks of forced endurance training using a small-animal treadmill or sedentary age-matched control. Transcriptomic analysis of 8-week training hearts was evaluated using RNA sequencing. RNA sequencing revealed several genes involved in the cell cycle, apoptosis, contractile protein expression, and organ growth that were among the most differentially regulated genes after 8-weeks of exercise.

2025-12-02 | GSE308626 | GEO

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