Project description:To understand the molecular details of the biosynthesis, secretion, and/or mode of action of antimicrobials, we performed a comparative analysis of transcripts expressed during log and stationary phases of growth of in Xenorhabdus szentirmaii to identify the deferentially expressed genes. Total RNA was isolated from X. szentirmaii grown at log and stationary phases (Fig 3A, four biological replicates referred here Dey1, Dey2, Dey3 and Dey4 for the log phase and Dey5, Dey6, Dey7 and Dey8 for the stationary phase, Supplemental Table 1). The equal amount of total RNA was subjected to RNA sequencing analysis.
Project description:A fliZ mutant in the entomopathogenic bacterium X. nematophila is attenuated in virulence in the insect. The goal of this study is to compare transcriptomes of the fliZ mutant and wild type strain to identify the FliZ regulon.
