Project description:Generation of in vitro models of cancer stem cells for in vivo xenograft models. hiPSC differentiation into a desired tissue allows studying physiologic processes in human-relevantcell systems. By introducing disease-specific mutations, these cells allow studying genetic origins of diseases in a tissue-specific manner.
Project description:Generation of in vitro models of cancer stem cells for in vivo xenograft models. hiPSC differentiation into a desired tissue allows studying physiologic processes in human-relevantcell systems. By introducing disease-specific mutations, these cells allow studying genetic origins of diseases in a tissue-specific manner.
Project description:Generation of in vitro models of cancer stem cells for in vivo xenograft models. hiPSC differentiation into a desired tissue allows studying physiologic processes in human-relevantcell systems. By introducing disease-specific mutations, these cells allow studying genetic origins of diseases in a tissue-specific manner.
Project description:Human induced pluripotent stem cell (hiPSC)-derived endothelial cells were transplanted into mouse liver and isolated at several time-points to assess their developmental profile over time. They were compared to in vitro hiPSC-endothelial cells, and primary human liver sinusoidal endothelial cells (either freshly isolated or expanded in culture).
Project description:Metabolic dysfunction-associated steatohepatitis (MASH) and liver fibrosis represent distinct yet interconnected pathological processes underlying chronic liver disease progression. While high-fat diet (HFD)-induced MASH models recapitulate metabolic inflammation, carbon tetrachloride (CCl₄)-induced models capture toxicant-driven fibrogenesis. Here, we performed single-cell RNA sequencing (scRNA-seq) on liver tissues from HFD-induced MASH and CCl₄-induced fibrosis mouse models to construct a high-resolution atlas of liver cellular heterogeneity. This work establishes a valuable resource for dissecting liver disease mechanisms, enabling targeted therapeutic discovery and offering a framework for integrating diverse preclinical models of chronic liver injury.