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RNA-seq of whole ileums from wildtype mice that were either exposed or not with diets with different protein concentrations for 4 weeks

ABSTRACT: RNA-seq of whole ileums from wildtype mice that were either exposed or not with diets with different protein concentrations for 4 weeks

PROVIDER: PRJEB49522 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR7756310_1.fastq.gz	Fastqsanger.gz
	ERR7756310_2.fastq.gz	Fastqsanger.gz
	ERR7756320_1.fastq.gz	Fastqsanger.gz
	ERR7756320_2.fastq.gz	Fastqsanger.gz
	ERR7756311_1.fastq.gz	Fastqsanger.gz

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RNA-seq of whole ileums from wildtype mice that were either exposed or not with diets with different protein concentrations for 4 weeks

Project description:We found that low protein diet consumption for 3 weeks resulted in reduction in the Paneth cells and goblet cells and villous height in wild type mice, indicating that low protein diet could negatively affect gut pathology. We performed transcriptional profiling to identify dysregulated molecular pathways in wild type mice exposed to low diet. Male mice were used at 4-5 weeks of age. The full-thickness ileum of the mice were harvested for transcriptomic analysis after completing 4 weeks of low protein diet treatment.

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RNA-seq of full thickness small intestine from Atg16l1 T300A and wildtype littermate mice that were either exposed or not with mainstream cigarette smoking for 6 weeks

Project description:We found that mainstream cigarette smoking (4 cigarettes/day, 5 days/week for 6 weeks using Kentucky Research Cigarettes 3R4F) resulted in >20% decrease in the percentage of normal Paneth cell population in Atg16l1 T300A mice but showed minimal effect in wildtype littermate control mice, indicating that Atg16l1 T300A polymorphism confers sensitivity to cigarette smoking-induced Paneth cell damage. We performed transcriptional profiling to identify molecular mechanisms associated with Paneth cell defect in Atg16l1 T300A mice exposed to cigarette smoking. Female mice were used at 4-5 weeks of age. Cigarette smoking was performed using smoking chamber with the dosage and schedule as described above. The whole ileum of the mice were harvested for transcriptomic analysis after completing 6 weeks of smoking.

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