Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NTRAS hnRNPL RNA sequencing in HUVECs

ABSTRACT: NTRAS hnRNPL RNA sequencing in HUVECs

PROVIDER: PRJEB49865 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR7925572.fastq.gz	Fastqsanger.gz
	ERR7925573.fastq.gz	Fastqsanger.gz
	ERR7925574.fastq.gz	Fastqsanger.gz
	ERR7925575.fastq.gz	Fastqsanger.gz
	ERR7925576.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

NTRAS hnRNPL RNA sequencing in HUVECs

Project description:In the current project with aim to unequivocally characterize a novel splicing-regulatory network that proves to be a central mediator of endothelial barrier function and vascular integrity. At the core of this network is the endothelial enriched lncRNA NTRAS (annotated as RP11-354k1.1) is shown to control alternative splicing decisions in HUVECs through interplaying with splicing factor hnRNPL. Specifically, in the project we show that NTRAS sequesters the splicing factor hnRNPL through a CA dinucleotide motif, to enhance TJP1 exon 20 usage, thereby TJP1α+ isoform. In turn disrupting TJP1α+ isoform expression impaired endothelial barrier function. Collectively, this splicing-regulatory network might prove fundamental in unlocking new interventions strategic to prevent or reverse vascular leakage.

2022-01-10 | E-MTAB-11311 | biostudies-arrayexpress

hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer (hnRNPL ChIPseq)

Project description:Ovarian cancer has the highest mortality rate among gynecologic tumors worldwide, with unclear underlying mechanisms of pathogenesis. RNA-binding proteins (RBPs) primarily direct post-transcriptional regulation through modulating RNA metabolism. Recent evidence demonstrates that RBPs are also implicated in transcriptional control. However, the role and mechanism of RBP-mediated transcriptional regulation in tumorigenesis remain largely unexplored. Here, we show that the RBP heterogeneous ribonucleoprotein L (hnRNPL) interacts with chromatin and regulates gene transcription by forming phase-separated condensates in ovarian cancer. hnRNPL phase separation activates PIK3CB transcription and glycolysis, thus promoting ovarian cancer progression. Notably, we observe that the PIK3CB promoter is transcribed to produce a non-coding RNA which interacts with hnRNPL and promotes hnRNPL condensation. Furthermore, hnRNPL is significantly amplified in ovarian cancer, and its high expression predicts poor prognosis for ovarian cancer patients. By using cell-derived xenograft and patient-derived organoid models, we show that hnRNPL knockdown suppresses ovarian tumorigenesis. Together, our study reveals that phase separation of the chromatin-associated RBP hnRNPL promotes PIK3CB transcription and glycolysis to facilitate tumorigenesis in ovarian cancer. The formed hnRNPL-PIK3CB-AKT axis depending on phase separation can serve as a potential therapeutic target for ovarian cancer.

2025-02-13 | GSE289074 | GEO

RNA sequencing of Opa1-silenced HUVECs.

Project description:RNA sequencing of Opa1-silenced HUVECs.

| PRJNA503252 | ENA

RNA sequencing for HUVECs treated with si-FLRT2

Project description:RNA sequencing for HUVECs treated with si-control or si-FLRT2

2022-01-22 | GSE179836 | GEO

hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer (hnRNPL RNAseq)

2025-02-14 | GSE289102 | GEO

HNRNPL RIP-seq in LNCaP prostate cancer cells

Project description:RNA immunoprecipitation coupled with next generation sequencing (RIP-seq) was used to map the HNRNPL-RNA interactome in LNCaP cells.

2016-12-28 | GSE72841 | GEO

RNA sequencing for HUVECs treated with si-FLRT2

Project description:RNA sequencing for HUVECs treated with si-FLRT2

| PRJNA745197 | ENA

small RNA sequencing of human endothelial cells (HUVECs)

Project description:small RNA sequencing of human endothelial cells (HUVECs)

| PRJNA414622 | ENA

Transcriptome sequencing of HUVECs

Project description:Transcriptome sequencing of HUVECs

| PRJNA649251 | ENA

hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer (RNApolII ChIPseq)

2025-02-13 | GSE289075 | GEO