RNA-Seq data from dairy goat milk somatic cells in response to fish oil diet induced milk fat depression
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB51882 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| ERR9390110_1.fastq.gz | Fastqsanger.gz | |||
| ERR9390110_2.fastq.gz | Fastqsanger.gz | |||
| ERR9390130_1.fastq.gz | Fastqsanger.gz | |||
| ERR9390130_2.fastq.gz | Fastqsanger.gz |
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Project description:This study aimed to characterize the whey proteome of colostrum, transitional milk, and mature milk from Murciano-Granadina goats, and to evaluate the use of transitional milk as a substitute for colostrum in feeding neonatal kids. Proteomic analysis identified a total of 1,385 proteins, with 736 in colostrum, 668 in transitional milk, and 625 in mature milk. Colostrum and transitional milk displayed a high degree of similarity between colostrum and transitional milk, particularly in proteins related to immune response and inflammation. In contrast, mature milk showed a shift toward proteins involved in metabolic and transport functions. To evaluate the practical application of transitional milk, newborn goat kids were fed either frozen colostrum (following standard farm protocol), freeze-dried colostrum, or freeze-dried transitional milk. All feeding strategies were well tolerated, with no adverse health effects, and resulted in similar growth performance. At 48 hours of life, serum IgG concentrations were 13.95 mg/mL in kids fed frozen colostrum, 17.36 mg/mL in those fed freeze-dried colostrum and 11.65 mg/mL in those fed freeze-dried transitional milk. Notably, kids fed freeze-dried transitional milk achieved satisfactory immunoglobulin levels, supporting its potential for passive immune transfer. These results highlight the biological value of transitional milk and support its use as a colostrum substitute in intensive farming systems, particularly when maternal colostrum is unavailable or insufficient. Furthermore, freeze-drying effectively preserved its functional properties, enabling convenient storage, handling, and administration. Overall, the successful use and preservation of transitional milk represent a sustainable approach to improving colostrum management, reducing waste, and increasing the efficiency of kid rearing in dairy goat production, in line with circular economy principles.
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Project description:This investigation reports a differential proteomic analysis of the goat milk to evaluate and understand the protein changes induced by Staphylococcus spp. during a subclinical intramammary infection (IMI). A number of 9 milk samples from multiparous goats were selected for the study, 3 of which in mid-lactation (30-60 Days in milk - DIM) with very low somatic cell count (SCC) from half-udders producing a sterile milk bacterial culture (MLU), 3 of which in late lactation samples (> 250 DIM) with SCC > 2,000,000 cells/mL from half-udders producing a sterile milk bacterial culture for the whole lactation (LHU) and in conclusion 3 in late lactation (> 250 DIM) with SCC > 2,000,000 cells/mL from goat half-udders with a milk bacterial culture repeatedly positive for Staphylococcus aureus (LHS). Samples were analyzed using a shotgun proteomics approach, based on filter-aided sample preparation (FASP) followed by LC-MS/MS and on differential analysis conducted by spectral count approach (1). (1) Pisanu S, Cacciotto C, Pagnozzi D, Puggioni GMG, Uzzau S, Ciaramella P, Guccione J, Penati M, Pollera C, Moroni P, Bronzo V, Addis MF. Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci. Sci Rep, 2019, 9 (1), 15850. Here, we reported both a characterization of goat milk proteins and a panel of differential proteins specific of S. Aureus-infected milk that mostly affected proteins involved in defense response processes and cytoskeleton organization. In conclusion, our results provide a depth characterization of milk proteins in goat samples uninfected and infected with S. Aureus, describe the changes induced by LHU and LHS subclinical intramammary infection and suggest indications to reveal subclinical staphylococcal mastitis in goat by a proteomic investigation of milk.
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Project description:Mammary epithelial cells (MECs) have strong secretory function, which is an important target cell for studying mammary gland bioreactor. It is also a unique cell with lactation function in mammary gland. It can be used to study cell biological characteristics and molecular biological mechanism in the process of mammary gland growth, development and lactation. At present, mammary epithelial cells are obtained from mammary tissue, and the extraction of tissue block involves surgical anesthesia, which may lead to breast tissue injury and infection. This study aimed to describe the process of isolating and culturing goat mammary epithelial cells (GMECs) from goat milk, and to identify the biological characteristics of GMECs. Our study laid a foundation for the study of the regulation mechanism of mammary gland development and lactation, the improvement of milk quality, the increase of milk production and the establishment of mammary gland bioreactor.
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