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Single-nucleus multiome (RNA+ATAC) sequencing of the axolotl pallium

ABSTRACT: Single-nucleus multiome (RNA+ATAC) sequencing of the axolotl pallium

PROVIDER: PRJEB52517 | ENA |

REPOSITORIES: ENA

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Single-nucleus multiome (RNA+ATAC) sequencing of the axolotl pallium

Project description:In order to understand the genomic and transcriptomic variability of the axolotl pallium, as well as reconstruct their intrinsic gene regulatory networks, we performed single-nucleus multiome sequencing (RNA and open chromatin) of whole axolotl pallium.

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Div-seq of axolotl pallium injury time course

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Multiome RNA+ATAC on human PBMCs (snATAC-seq data)

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Project description:This study generated a single-nucleus multiomic dataset of the adult cerebellar cortex across four anthropoid primate species: Homo sapiens (n=4), Pan troglodytes (n=3), Macaca mulatta (n=4), and Callithrix jacchus (n=4). Paired single-nucleus RNA sequencing and chromatin accessibility profiling were performed using the 10x Genomics Chromium Multiome platform. After quality control, 69,302 nuclei were retained for RNA analysis and 63,491 nuclei for ATAC analysis, with 60,824 nuclei profiled in both modalities. The dataset provides single-nucleus transcriptomic and chromatin accessibility profiles for comparative analysis of cerebellar cell types across primates.

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