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Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

ABSTRACT: No sensing of rAAV8 by human monocyte-derived dendritic cells

PROVIDER: PRJEB53021 | ENA |

REPOSITORIES: ENA

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Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

Project description:Recombinant Adeno-Associated Virus (rAAV) is considered as one of the most successful and widely used viral vector for in vivo gene therapy. However, host immune responses to the vector and/or the transgene product remain a major hurdle to successful AAV gene transfer. In contrast to anti-vector adaptive immunity, the initiation of the innate immunity towards rAAV is still poorly understood but is directly dependent on the interaction between the viral vector and innate immune cells. Here, we used a quantitative transcriptomic-based approach to determine the activation of inflammatory and anti-viral pathways after rAAV8-based infection of monocyte-derived Dendritic Cells (moDC) obtained from 12 healthy human donors. We have shown that rAAV8 particles are efficiently internalized, but that this uptake does not induce any detectable transcriptomic change in moDC cells in contrast to an adenoviral infection which upregulates anti-viral pathways. These findings suggest an immunological favorable profile for rAAV8 serotype with regards to in vitro activation of moDC cell model. Transcriptomic analysis of rAAV-infected innate immune cells is a powerful method to determine the ability of the viral vector to be seen by these sensor cells, which remains of great importance to better understand the immunogenicity of rAAV vectors and to design immune-stealth products.

2023-09-12 | GSE228649 | GEO

Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells.

Project description:Recombinant Adeno-Associated Virus (rAAV) is considered as one of the most successful and widely used viral vectors for in vivo gene therapy. However, host immune responses to the vector and/or the transgene product remain a major hurdle to successful AAV gene transfer. In contrast to antivector adaptive immunity, the initiation of the innate immunity towards rAAV is still poorly understood but is directly dependent on the interaction between the viral vector and innate immune cells. Here, we used a quantitative transcriptomic-based approach to determine the activation of inflammatory and anti-viral pathways after rAAV8-based infection of monocyte-derived dendritic cells (moDCs) obtained from 12 healthy human donors. We have shown that rAAV8 particles are efficiently internalized, but that this uptake does not induce any detectable transcriptomic change in moDCs in contrast to an adenoviral infection, which upregulates anti-viral pathways. These findings suggest an immunologically favorable profile for rAAV8 serotype with regard to in vitro activation of moDC model. Transcriptomic analysis of rAAV-infected innate immune cells is a powerful method to determine the ability of the viral vector to be seen by these sensor cells, which remains of great importance to better understand the immunogenicity of rAAV vectors and to design immune-stealth products.

| S-EPMC10341499 | biostudies-literature

Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

Project description:rAAV8 does not activate human monocyte-derived Dendritic Cells

| PRJEB53312 | ENA

Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

Project description:rAAV8 does not activate human monocyte-derived Dendritic Cells

| PRJEB54612 | ENA

Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

Project description:Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells

| PRJNA950939 | ENA

Transcriptomic profiling of control and CBD treated monocyte-derived dendritic cells

Project description:Transcriptomic profiling of control and CBD treated monocyte-derived dendritic cells

2023-08-13 | GSE235310 | GEO

Monocyte Derived Dendritic Cell Maturation

Project description:Human monocyte derived dendritic cells matured via galectin-1 or LPS. Keywords: dendritic cell maturation

2006-08-02 | GSE4984 | GEO

Quantitative analysis of large-scale proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study

Project description:Determination of global alterations in monocyte-derived dendritic cells in response to stimulation with lipopolysaccharide using SWATH-MS to provide insight into cellular mechanism driving dendritic cell maturation

2019-03-18 | PXD008898 | Pride

Transcriptome profiles of mouse and human monocyte and dendritic cell subsets

Project description:This SuperSeries is composed of the following subset Series: GSE35457: Transcriptome profiles of mouse and human monocyte and dendritic cell subsets (human data) GSE35458: Transcriptome profiles of mouse and human monocyte and dendritic cell subsets (mouse data) Refer to individual Series

2012-07-11 | E-GEOD-35459 | biostudies-arrayexpress

Comparison of human plasmacytoid dendritic cells and monocyte-derived dendritic cells

Project description:Comparison of human plasmacytoid dendritic cells and monocyte-derived dendritic cells

| PRJEB66498 | ENA