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RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations

ABSTRACT: RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations

PROVIDER: PRJEB53526 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9847080_1.fastq.gz	Fastqsanger.gz
	ERR9847080_2.fastq.gz	Fastqsanger.gz
	ERR9847071_1.fastq.gz	Fastqsanger.gz
	ERR9847071_2.fastq.gz	Fastqsanger.gz
	ERR9847081_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations

Project description:RNA-seq of Arabidopsis thaliana plants (Col-0) treated with putrescine (100 µM), spermine (100 µM), flg22 (1 µM) and combinations (putrescine + flg22; spermine + flg22)

2022-10-20 | E-MTAB-11820 | biostudies-arrayexpress

Mass spectrometry distinguishes putrescine, spermidine, and spermine covalently attached to proteins

Project description:Bacterial and mammalian cells are rich in putrescine, spermidine and spermine. Polyamines can be incorporated into proteins in vitro. Very few naturally occurring polyaminated proteins have been identified. Bovine albumin and the recombinant universal stress protein from Francisella tularensis were used as models for mass spectrometry analysis of polyaminated proteins. The proteins were covalently bound to putrescine, spermidine, or spermine by the action of carbodiimide or microbial transglutaminase. Tryptic peptides were subjected to liquid chromatography tandem mass spectrometry. Adducts were identified by Protein Prospector software. We describe the search parameters for identifying polyaminated peptides in Protein Prospector and show convincing MMS spectra for adducts with putrescine, spermidine, and spermine. Manual evaluation led us to recognize signature ions for polyamine adducts on Asp, Glu, and Gln. Manual evaluation recognized neutral loss from putrescine, spermidine and spermine during the fragmentation process. Mechanisms for formation of signature ions and neutral loss are presented. Manual evaluation recognized a false positive adduct which had been formed during trypsinolysis by rearrangement of a peptide sequence. Another false positive was a 71 Da added mass on cysteine, which was initially assumed to be putrescine, but was actually a propionamide adduct for a sample extracted from a polyacrylamide gel. The type of information presented in this report serves as a model for identifying naturally occurring polyaminated proteins.

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Transcriptional analysis of polyamine responsive genes

Project description:Genes differentially expressed by exogenously supplied polyamines: putrescine, spermidine, spermine, thermospermine and cadaverine in Arabidopsis thaliana.

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Microarray data of differentiated human keratinocytes treated with and without AMD1 inhibitor EGBG and rescued with spermidine/ spermine.

Project description:Maintaining tissue homeostasis depends on a balance of cell proliferation, differentiation and apoptosis. Polyamine regulator, AMD1, is a crucial regulator of keratinocyte differentiation and AMD1 protein is upregulated on differentiation and highly expressed in the suprabasal layers of the human epidermis. During keratinocyte differentiation, elevated AMD1 promotes decreased putrescine and increased spermine levels. Inhibition of AMD1 results in reduced spermine levels and inhibition of keratinocyte differentiation. Supplementing AMD1 inhibited keratinocytes with exogenous spermidine/spermine rescued aberrant differentiation. Undifferentiated and differentiated keratinocytes that had been treated with and without AMD1 inhibitor EGBG in the presence or absence of spermidine/ spermine supplementation were subjected to microarray analysis. These data show that AMD1 up regulation is required for keratinocyte differentiation and that inhibition of AMD1 can be rescued by supplementation with the polyamines spermidine and spermine.

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Flg22 induces CERK1 phosphorylation

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