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Metabolic reconstitution by a gnotobiotic microbiota varies over the circadian cycle

ABSTRACT: Metabolic reconstitution by a gnotobiotic microbiota varies over the circadian cycle

PROVIDER: PRJEB53981 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9897580_1.fastq.gz	Fastqsanger.gz
	ERR9897580_2.fastq.gz	Fastqsanger.gz
	ERR9897590_1.fastq.gz	Fastqsanger.gz
	ERR9897590_2.fastq.gz	Fastqsanger.gz
	ERR9897600_1.fastq.gz	Fastqsanger.gz

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Project description:The capacity of the intestinal microbiota to degrade otherwise indigestible diet components is known to greatly improve the recovery of energy from food. This has led to the hypothesis that increased digestive efficiency may underlie the contribution of the microbiota to obesity. OligoMM12-colonized gnotobiotic mice have a consistently higher fat mass than germ-free (GF) or fully colonized counterparts. We therefore investigated their food intake, digestion efficiency, energy expenditure, and respiratory quotient using a novel isolator-housed metabolic cage system, which allows long-term measurements without contamination risk. This demonstrated that microbiota-released calories are perfectly balanced by decreased food intake in fully colonized versus gnotobiotic OligoMM12 and GF mice fed a standard chow diet, i.e., microbiota-released calories can in fact be well integrated into appetite control. We also observed no significant difference in energy expenditure after normalization by lean mass between the different microbiota groups, suggesting that cumulative small differences in energy balance, or altered energy storage, must underlie fat accumulation in OligoMM12 mice. Consistent with altered energy storage, major differences were observed in the type of respiratory substrates used in metabolism over the circadian cycle: In GF mice, the respiratory exchange ratio (RER) was consistently lower than that of fully colonized mice at all times of day, indicative of more reliance on fat and less on glucose metabolism. Intriguingly, the RER of OligoMM12-colonized gnotobiotic mice phenocopied fully colonized mice during the dark (active/eating) phase but phenocopied GF mice during the light (fasting/resting) phase. Further, OligoMM12-colonized mice showed a GF-like drop in liver glycogen storage during the light phase and both liver and plasma metabolomes of OligoMM12 mice clustered closely with GF mice. This implies the existence of microbiota functions that are required to maintain normal host metabolism during the resting/fasting phase of circadian cycle and which are absent in the OligoMM12 consortium.

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Metabolic reconstitution by a gnotobiotic microbiota varies over the circadian cycle

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