Wairau Bar
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REPOSITORIES: ENA
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Project description:Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops. Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibit substrate preference toward phosphinothricin over the 20 proteinogenic amino acids, indirect effects of BAR-containing transgenes in planta, including modified amino acid levels, have been seen but without the identification of their direct causes. Combining metabolomics, plant genetics, and biochemical approaches, we show that transgenic BAR indeed converts two plant endogenous amino acids, aminoadipate and tryptophan, to their respective N-acetylated products in several plant species examined. We report the crystal structures of BAR, and further delineate structural basis for its substrate selectivity and catalytic mechanism. Through structure-guided protein engineering, we generated several BAR variants that display significantly reduced nonspecific activities compared to its wild-type counterpart in vivo. Our results demonstrate that transgenic expression of enzymes can result in unintended off-target metabolism arising from enzyme promiscuity. Understanding of such phenomena at the mechanistic level can facilitate the design of maximally insulated systems featuring heterologously expressed enzymes.
2017-12-05 | MTBLS553 | MetaboLights
Project description:We investigated the changes of gene expression in PHA-producing Pseudomonas putida KT2440 cultivated under elevated pressure (7 bar) and under combined elevated pressure (7 bar) and elevated dissolved oxygen tension by means of DNA microarrays. RNA samples were isolated from cells cultivated in chemostat under very well defined growth conditions (growth rate, medium, temperature, pH,...)
2012-12-03 | E-MEXP-3632 | biostudies-arrayexpress
Project description:Pneumococcal meningitis outbreak in BAR Ghana 2016
| PRJEB15437 | ENA
Project description:Miscanthus sinensis Gold Bar EF0701 genome sequencing
| PRJNA337654 | ENA
Project description:Aeromonas dhakensis strain:BAR-OCT | isolate:Environmental Genome sequencing
| PRJNA1211687 | ENA
Project description:BAR-ChIP on yeast chromatin associated mutants
| PRJEB7324 | ENA
Project description:BAR-Seq to study history-dependent behavior
| PRJNA478858 | ENA
Project description:Background Recurrent spontaneous abortion (RSA) remains a formidable clinical challenge with limited therapeutic options, largely attributed to trophoblast dysfunction as a central pathological mechanism. Bushen Antai recipe (BAR) has demonstrated encouraging clinical and experimental efficacy in pregnancy support; however, its bioactive constituents and molecular mechanisms, especially in the context of trophoblasts, remain poorly understood. Purpose To evaluate the therapeutic efficacy of BAR in RSA and elucidate its underlying mechanisms, with a particular focus on trophoblast function, using an integrated strategy. Methods UPLC-Q-TOF-MS/MS was employed to profile chemical constituents of BAR and its serum-absorbed components. An RU486-induced abortion-prone mouse model and HTR-8/SVneo trophoblast injury model were established to evaluate BAR's effects on embryonic development and placentation. Label-free quantitative proteomics, network pharmacology, molecular docking, viral transduction, together with transmission electron microscopy imaging, morphological analysis, western blot, immunofluorescence, and various cellular functional assays were performed to decipher the underlying mechanisms. Results BAR significantly reduced embryo resorption, restored placental architecture, and improved trophoblast survival and function in abortion-prone mice. Proteomics revealed endoplasmic reticulum (ER) stress as a central pathological feature of RSA placentas, which was effectively ameliorated by BAR treatment. Network pharmacology predicted VEGFA as a core target, subsequently validated by experiments demonstrating that BAR activates the VEGFA/p-AKT/SESN2 axis to reprogram the dynamics of all three unfolded protein response (UPR) branches and re-establish ER homeostasis. Serum pharmacochemistry identified 72 absorbed constituents, with molecular docking revealing that multiple compounds including quercetin, calycosin, loganin, and sweroside may directly target VEGFA.
2026-03-07 | PXD075381 |
Project description:The first gut metagenome of wild Bar-headed geese
| PRJEB12153 | ENA