Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of the transcriptome profile of bursa of Fabricius infected by Muscovy duck reovirus in muscovy ducklings

ABSTRACT: Characterization of the transcriptome profile of bursa of Fabricius infected by Muscovy duck reovirus in muscovy ducklings

PROVIDER: PRJEB55500 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR10097277.fastq.gz	Fastqsanger.gz
	ERR10097278.fastq.gz	Fastqsanger.gz
	ERR10097279.fastq.gz	Fastqsanger.gz
	ERR10097280.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 27

Similar Datasets

Comparative proteomic analysis revealed the differential responses to novel duck reovirus infection in the bursa of Fabricius of Cairna moschata

Project description:Duck reovirus (DRV) is well-studied aquatic bird virus belonging to the Orthoreovirus genus of the Reoviridae family. The bursa of Fabricius is an immunologically organ against virus invasion. However, the responses of the bursa of Fabricius of Cairna moschata to DRV infection are largely unknown. To investigate the immune responses, the proteomes from the control and two DRV strain infected samples (NH and DJ) were compared. In total, 7075 protein were identified, of which 5625 protein were quantified. A number of differentially expressed proteins (DEPs), including 210 DEPs under the HN10 infection and 55 DEPs under the JD10 infection, were identified. Protein network analysis showed that the DEPs enriched in the serine protease system and the innate immune response clusters. For the serine protease systems, coagulation factor IX, three chains of fibrinogen, and complement C8, C5, and C2s were significantly up-regulated, suggesting that the serine protease-mediated immune might be involved in the responses to the HN10 infection. For the innate and adaptive immune system, RIG-I, MDA5, MAPK20, and IRF3 were significantly up-regulated, indicating their important role in the reorganization of invaded virus. Furthermore, the DEPs among different visceral organs (liver, spleen, and the bursa of Fabricius) were compared. coagulation factor IX was significantly up-regulated in the bursa of fabricius, not in the liver and spleen samples, suggesting an important role of the bursa of fabricius in antivirus. Our data may give a comprehensive resource for investigating the regulation mechanism involved in the responses of the bursa of Fabricius of duck to the DRV infections.

2022-08-12 | PXD025093 | Pride

Characterization of the transcriptome profile of bursa of Fabricius infected by Muscovy duck reovirus in muscovy ducklings

Project description:Whole transcriptome sequencing was carried on to investigate the different response of bursa of Fabricius of ducklings to HN10 (virulent NDRV) and JDm10 (naturally attenuated NDRV).

2022-08-29 | E-MTAB-12115 | biostudies-arrayexpress

Enrichment of m6A and G3BP1 on mRNA of bursa of Fabricius of Muscovy ducklings infected by HN10 NDRV

Project description:one-day-old healthy Muscovy ducklings were collected and randomly separated into groups (n = 10 for each group). Ducklings were administered 500 μl of the HN10 strain at a titer of 106.4 TCID50/ml for NDRV infection, whereas sterile DMEM was used as a negative control (NC). The bursa of Fabricius specimens was harvested at 72 hours postinfection (hpi). RNA was isolated, and IP was performed to enrich m6A modified RNA and G3BP1 bound RNA respectively.

2024-11-15 | E-MTAB-14565 | biostudies-arrayexpress

Abdominal fat mRNA transcriptome of Muscovy duck

Project description:Abdominal fat is considered negatively associated with egg production. Therefore, it is necessary to clarity the regulatory mechanism for the abdominal fat deposition of Muscovy duck.

2021-02-04 | GSE144727 | GEO

Gene expression of bursa fabricius of Thai indigenous chicken breeds

Project description:Analysis of gene expression of Bursa of Fabricius samples of 3 male chickens of 6 breeds revealed differential expression of genes related to Ingenuity pathways immune cell trafficking, cell-mediated immune response, humoral immune response and infectious disease.

2018-12-31 | GSE107114 | GEO

Distinct Whole Transcriptomic Profiles of the Bursa of Fabricius in Muscovy Ducklings Infected by Novel Duck Reovirus with Different Virulence.

Project description:Novel duck reovirus (NDRV) is a newly identified reovirus that brings about more severe damage on multiple organs and mortality in various species of waterfowl. We previously characterized the transcriptomic profiles responding to NDRV in the bursa of Fabricius of Muscovy ducklings, which is a major immunological organ against virus infection. However, the molecular mechanisms of variant cell responses in the bursa of Fabricius to NDRV with different virulence is unclear. Here, we conducted a whole transcriptomic analysis to study the effects of two strains, HN10 (virulent NDRV) and JDm10 (artificially attenuated NDRV), on the bursa of Fabricius of Muscovy ducklings. We harvested a large number of differentially expressed genes (DEGs) of the bursa of Fabricius specially induced by HN10 and JDm10, and we found that HN10 induced DEGs enriched in differentiation and development in multiple organs beyond JDm10. Moreover, the ceRNA regulatory network also indicated the different connections among mRNA, lncRNA and miRNA. Interestingly, we further noticed that a population of differential expressed miRNA could particularly target to transcripts of HN10 and JDm10. We took miR-24 as an example and observed that miR-24 could reduce the transcription of GLI family zinc finger 3 (Gli3) and membrane-associated guanylate kinase, WW and PDZ domain containing 1 (Magi1) via recognition 3' UTR of these two genes by a dual luciferase reporter gene assay in vitro. However, this effect could be compromised by HN10 infection or the ectopic over-expression of the putative miR-24 targeting regions in L1 and L3 fragments of HN10. Taken together, we examined and proposed a novel regulatory competitive mechanism between transcripts of NDRV and Muscovy ducklings for miRNA. These findings may advance the understanding of the molecular pathogenesis of NDRV in Muscovy ducklings, and help provide the potential targets for vaccine and drug development against NDRV.

| S-EPMC9866435 | biostudies-literature

Anas platyrhynchos breed:Jinding duck, Muscovy duck

Project description:Anas platyrhynchos breed:Jinding duck, Muscovy duck Raw sequence reads

| PRJNA874907 | ENA

RNA-seq of mRNAs from spleen and bursa of Fabricius of white leghorn chickens (specific pathogen-free)

Project description:We have used RNA-seq to examine mRNAs from chicken spleen and bursa of Fabricius of three different condition (non-immunized and non-heat-stressed (24 ± 1℃ for 3 h), immunized (Newcastle disease vaccine) and non-heat-stressed, and immunized and heat-stressed (36 ± 1℃ for 3 h)). To clarify how chicken immune systems responded to heat stress with and without immunization.

2018-12-01 | GSE93224 | GEO

Muscovy duck ileum microorganism

Project description:Muscovy duck ileum microorganism Genome sequencing and assembly

| PRJNA690229 | ENA

Transcription profiling of bursa of Fabricius from genetically resistant and susceptible chickens infected with Infectious Bursal Disease Virus (IBDV)

Project description:We used a chicken immune-targeted gene array to analyse the differences in gene expression in the bursa of Fabricius from genetically resistant and susceptible animals infected with Infectious Bursal Disease Virus (IBDV).

2006-10-19 | E-MEXP-756 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data