Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing bacteria
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PROVIDER: PRJEB56267 | ENA |
REPOSITORIES: ENA
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Project description:We report a new DSR pathway that directly generates zero-valent sulfur (ZVS) in phylogenetically diverse SRB.
2023-04-27 | E-MTAB-12239 | biostudies-arrayexpress
Project description:Dissimilatory sulfate reduction (DSR) mediated by sulfate-reducing microorganisms (SRMs) plays a pivotal role in global sulfur, carbon, oxygen, and iron cycles since ~3.5 billion years ago. The canonical DSR pathway is believed to be sulfate reduction to sulfide. Herein, we report a new DSR pathway in phylogenetically diverse SRMs through which zero-valent sulfur (ZVS) is directly generated. We identified that approximately 8.9% of sulfate reduction was directed toward ZVS with S8 as a predominant product, and the ratio of sulfate-to-ZVS could be changed with SRMs’ growth conditions, particularly the medium salinity. Further coculturing experiments and metadata analyses revealed that DSR-derived ZVS supported the growth of various ZVS-metabolizing microorganisms, highlighting this new pathway as an essential component of the sulfur biogeochemical cycle
2023-04-28 | PXD040825 | Pride
Project description:Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing bacteria
| PRJEB56466 | ENA
Project description:Production of zero valent sulfur from dissimilatory sulfate reduction in a methanogenic bioreactor
| PRJEB29929 | ENA
Project description:Dissimilatory sulfate reduction (DSR) mediated by sulfate-reducing microorganisms (SRMs) plays a pivotal role in global sulfur, carbon, oxygen, and iron cycles since at least 3.5 billion y ago. The canonical DSR pathway is believed to be sulfate reduction to sulfide. Herein, we report a DSR pathway in phylogenetically diverse SRMs through which zero-valent sulfur (ZVS) is directly generated. We identified that approximately 9% of sulfate reduction was directed toward ZVS with S8 as a predominant product, and the ratio of sulfate-to-ZVS could be changed with SRMs' growth conditions, particularly the medium salinity. Further coculturing experiments and metadata analyses revealed that DSR-derived ZVS supported the growth of various ZVS-metabolizing microorganisms, highlighting this pathway as an essential component of the sulfur biogeochemical cycle.
| S-EPMC10194018 | biostudies-literature
Project description:The existence of light in various deep-sea environments has been well established. Our previous research found blue light promotes zero-valent sulfur (ZVS) production in Erythrobacter flavus 21-3, a bacterium isolated from the deep-sea cold seep. E. flavus 21-3 can convert thiosulfate to ZVS through a novel thiosulfate oxidation pathway comprising a thiosulfate dehydrogenase (TsdA) and two thiosulfohydrolases (SoxB). Using proteomic analysis, bacterial two-hybrid system and heterologous expression assays, we found that infrared light also promotes zero-valent sulfur (ZVS) production in E. flavus 21-3. The bacteriophytochrome (bphp) Ef2bphp-15570 autophosphorylated and activated GGDEF domain-containing protein D0Y83_RS00450 to produce c-di-GMP. Subsequently, the PilZ protein mPilZ-1753 bound to c-di-GMP and activated downstream sulfur oxidation pathways. During this process, polyphosphate kinase 2 (PPK2) affects the content of c-di-GMP by competing for GTP, thereby together c-di-GMP regulating ZVS production, as well as other metabolic processes in E. flavus 21-3. This study provides a novel insight into a deep-sea non-photosynthetic bacterium which sensing infrared light to regulate sulfur metabolism through a bacteriophytochrome photoreceptor, thus offering new understandings perspectives on microbial utilization of light energy.
2025-05-07 | PXD058843 | Pride
Project description:Bacteria isolated from diverse environments were found to sense blue light to regulate their biological functions. However, this ability of deep-sea bacteria has been studied rarely. In this study, we found serendipitously that blue light stimulated excess zero-valent sulfur (ZVS) production of E. flavus 21-3, which was isolated from the deep-sea cold seep and possessed a novel thiosulfate oxidation pathway. Its ZVS production responding to the blue light was mediated by a light-oxygen-voltage histidine kinase (LOV-1477), a diguanylate cyclase (DGC-2902), a PilZ protein (mPilZ-1753) and the key thiosulfate dehydrogenase (TsdA) in its thiosulfate oxidation pathway. Subsequently, the thiosulfohydrolase (SoxB-277) was found working with another SoxB (SoxB-285) and being as substitute for each other to generate ZVS. This study provided an example of deep-sea bacteria sensing blue light to regulate thiosulfate oxidation. Deep-sea blue light potentially helped these blue-light-sensing bacteria adapt harsh conditions by diversifying their biological processes.
2023-01-03 | PXD035435 | Pride
Project description:Microbial sulfur cycling and chemoautotrophy are focal points of research in cold seeps. However, limited culture-dependent and in-situ studies have described the biological features and ecological significance of chemoautotrophic sulfur-oxidizing bacteria. In this study, we isolated Guyparkeria hydrothermalis SP2, a thiosulfate/sulfide-oxidizing chemoautotrophic bacterium, from cold-seep sediment. Electron microscopy, Raman spectroscopy, and stoichiometry confirmed the efficient production of zero-valent sulfur (ZVS) by G. hydrothermalis SP2. Genomic, transcriptomic, and qRT-PCR analyses revealed its utilization of the Sox pathway for thiosulfate oxidation and the fccB gene for sulfide oxidation. Its chemoautotrophic capability mediated by the Calvin-Benson-Bassham (CBB) cycle was identified through isotopic and qRT-PCR analyses. In-situ studies demonstrated its ability to produce ZVS by oxidizing sulfide in cold seeps, with a preference for different genes compared to those under laboratory conditions. Metagenomic and metatranscriptomic analyses indicated the ubiquity of its sulfur oxidation-based chemoautotrophic pathway in cold seep sediments. Therefore, this strain holds significance for investigating sulfur oxidation-based chemoautotrophic pathways in cold seeps.
2026-02-25 | PXD073871 | Pride
Project description:Sulfate often behaves conservatively in the oxygenated environments but serves as an electron acceptor for microbial respiration in a wide range of natural and engineered systems where oxygen is depleted. As a ubiquitous anaerobic dissimilatory pathway, therefore, microbial reduction of sulfate to sulfide has been of continuing interest in the field of microbiology, ecology, biochemistry, and geochemistry. Stable isotopes of sulfur are an effective tool for tracking this catabolic process as microorganisms discriminate strongly against heavy isotopes when cleaving the sulfur-oxygen bond. Along with its high preservation potential in environmental archives, a wide variation in the sulfur isotope effects can provide insights into the physiology of sulfate reducing microorganisms across temporal and spatial barriers. A vast array of parameters, including phylogeny, temperature, respiration rate, and availability of sulfate, electron donor, and other essential nutrients, has been explored as a possible determinant of the magnitude of isotope fractionation, and there is now a broad consensus that the relative availability of sulfate and electron donors primarily controls the magnitude of fractionation. As the ratio shifts toward sulfate, the sulfur isotope fractionation increases. The results of conceptual models, centered on the reversibility of each enzymatic step in the dissimilatory sulfate reduction pathway, are in qualitative agreement with the observations, although the underlying intracellular mechanisms that translate the external stimuli into the isotopic phenotype remain largely unexplored experimentally. This minireview offers a snapshot of our current understanding of the sulfur isotope effects during dissimilatory sulfate reduction as well as their potential quantitative applications. It emphasizes the importance of sulfate respiration as a model system for the isotopic investigation of other respiratory pathways that utilize oxyanions as terminal electron acceptors.
| S-EPMC10125365 | biostudies-literature
Project description:Molecular mechanism of zero valent iron-enhanced microbial azo reduction in Shewanella decolorationis S12
| PRJNA673394 | ENA