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RNAseq of iPSC derived pulmonary organoids by co-culturing endodermal and mesodermal progenitors

ABSTRACT: RNAseq of iPSC derived pulmonary organoids by co-culturing endodermal and mesodermal progenitors

PROVIDER: PRJEB56634 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10359586_1.fastq.gz	Fastqsanger.gz
	ERR10359586_2.fastq.gz	Fastqsanger.gz
	ERR10359587_1.fastq.gz	Fastqsanger.gz
	ERR10359587_2.fastq.gz	Fastqsanger.gz
	ERR10359588_1.fastq.gz	Fastqsanger.gz

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cDNA microarray Analysis of human iPSC-derived mesodermal progenitors treated with CHIR99021 compared to controls

Project description:The aim of this study was to unravel the effects of an initial WNT/β-catenin pulse on the in vitro differentiation of human iPSCs towards mesodermal progenitors and subsequent specification of chondrocytes. Therefore, mesodermal progenitors were generated from hiPSCs according to standard protocol (ctrl) or treated with 5 µM WNT/β-catenin agoinst CHIR99021 for 24h. To understand the long-term effects of short-initial WNT pulse on mesodermal differentiation, the global gene expression profile of day 14 mesodermal progenitors was compared.

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Project description:This study will analyse the global RNA expression patterns in human iPSC and endodermal differentiated cells.The goal of this project is to investigate genes regulating definitive endodermal differentiation of human iPSC using genetic screening. The differentiation of human iPSC into definitive endoderm is going to be examined by global RNA expression patterns. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2015-06-09 | E-ERAD-363 | biostudies-arrayexpress

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Project description:Muscular dystrophies (MDs) are often characterized by impairment of both skeletal and cardiac muscle. Regenerative strategies for both compartments therefore constitute a therapeutic avenue. Mesodermal iPSC-derived progenitors (MiPs) can regenerate both striated muscle types simultaneously in mice. Importantly, MiP myogenic propensity is influenced by somatic lineage retention. However, it is still unknown whether human MiPs have in vivo potential. Furthermore, methods to enhance the intrinsic myogenic properties of MiPs are likely needed, given the scope and need to correct large amounts of muscle in the MDs. Here, we document that human MiPs can successfully engraft into the skeletal muscle and hearts of dystrophic mice. Utilizing non-invasive live imaging and selectively induced apoptosis, we report evidence of striated muscle regeneration in vivo in mice by human MiPs. Finally, combining RNA-seq and miRNA-seq data, we define miRNA cocktails that promote the myogenic potential of human MiPs.

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2017-08-17 | GSE93524 | GEO

sibling aHDF-iPSC clones derived from the same fibroblasts

Project description:Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, little attention has so far been paid to variations among hiPSC lines in terms of their hepatic differentiation. We developed an improved hepatic differentiation protocol and compared multiple hiPSC lines. This comparison indicated that the hepatic differentiation propensity varies among sibling hiPSC clones derived from the same adult human dermal fibroblasts (aHDFs). In addition, hiPSC clones derived from peripheral blood cells (PB-iPSCs) consistently showed good hepatic differentiation efficiency, whereas many hiPSC clones from adult dermal fibroblasts (aHDF-iPSCs) showed poor hepatic differentiation. However, when we compared hiPSCs from blood and dermal fibroblasts from the same individuals, we found that variations in hepatic differentiation were largely attributable to donor differences, rather than to the types of the original cells. In order to understand the molecular mechanisms underlying the observed variations in hepatic differentiation, we performed microarray analyses of sibling aHDF-iPSC clones, and aHDF- and PB-iPSC clones from the same individuals. sibling aHDF-iPSC clones (201B6 and 201B7; derived from the same aHDFs) (1) undifferentiated state (n=4, biological replicate #1-#4) (2) CXCR4-positive cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4) (3) CXCR4-negative cell populations sorted by flowcytometry after 7 days of endodermal differentiation (n=4, biological replicate #1-#4)

2012-07-15 | E-GEOD-38152 | biostudies-arrayexpress

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