Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli

ABSTRACT: Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli

PROVIDER: PRJEB56816 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR10384401.fastq.gz	Fastqsanger.gz
	ERR10384402.fastq.gz	Fastqsanger.gz
	ERR10384403.fastq.gz	Fastqsanger.gz
	ERR10384404.fastq.gz	Fastqsanger.gz
	ERR10384405.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis

Project description:The experiment aimed to find how Campylobacter responds to oxidative stress using hydrogen peroxide. This was done by using previously made TraDIS libraries (https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02835-8) and putting them under oxidative stress. The strains used were C. jejuni 11168, C. coli 15-537360 and C. coli CCN182.

2023-09-14 | E-MTAB-13336 | biostudies-arrayexpress

Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli

Project description:Creation of Transposon libraries within 6 C. jejuni, 4 C. coli, 1 C. hyointestinalis and1 C. lari species. A mariner transposon was used to create libraries, these were sequenced and analysed using the Biotradis pipeline. This allowed us to investigate essential genes for each strain, across strains and between species.

2024-12-31 | E-MTAB-12314 | biostudies-arrayexpress

Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli

Project description:TraDIS in C. jejuni and C. coli

| PRJEB55469 | ENA

Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli.

Project description:Campylobacter species are the major cause of bacterial gastroenteritis. As there is no effective vaccine, combined with the rapid increase in antimicrobial resistant strains, there is a need to identify new targets for intervention. Essential genes are those that are necessary for growth and/or survival, making these attractive targets. In this study, comprehensive transposon mutant libraries were created in six C. jejuni strains, four C. coli strains and one C. lari and C. hyointestinalis strain, allowing for those genes that cannot tolerate a transposon insertion being called as essential. Comparison of essential gene lists using core genome analysis can highlight those genes which are common across multiple strains and/or species. Comparison of C. jejuni and C. coli, the two species that cause the most disease, identified 316 essential genes. Genes of interest highlighted members of the purine pathway being essential for C. jejuni whilst also finding that a functional potassium uptake system is essential. Protein-protein interaction networks using these essential gene lists also highlighted proteins in the purine pathway being major 'hub' proteins which have a large number of interactors across the network. When adding in two more species (C. lari and C. hyointestinalis) the essential gene list reduces to 261. Within these 261 essential genes, there are many genes that have been found to be essential in other bacteria. These include htrB and PEB4, which have previously been found as core virulence genes across Campylobacter species in other studies. There were 21 genes which have no known function with eight of these being associated with the membrane. These surface-associated essential genes may provide attractive targets. The essential gene lists presented will help to prioritise targets for the development of novel therapeutic and preventative interventions.

| S-EPMC10077673 | biostudies-literature

Campylobacter jejuni butyrate resopnse

Project description:Growth of Campylobacter jejuni in butyrate, including deletions of a TCS involved in sensing this response

2021-01-01 | GSE142852 | GEO

Diarrhoeal mechanisms of the Campylobacter jejuni enteritis

Project description:Campylobacter jejuni is the most prevalent cause of foodborne bacterial enteritis worldwide. This study aims at the characterisation of pathomechanisms and signalling in Campylobacter-induced diarrhoea in the human mucosa. During routine colonoscopy, biopsies were taken from patients suffering from campylobacteriosis. RNA-seq of colon biopsies was performed to describe Campylobacter jejuni-mediated effects. Mucosal mRNA profiles of acutely infected patients and healthy controls were generated by deep sequencing using Illumina HiSeq 2500. This data provide the basis for subsequent upstream regulator analysis.

2017-10-10 | GSE88710 | GEO

Campylobacter jejuni gene expression in germ-free mice

Project description:Expression arrays comparing Campylobacter jejuni NCTC11168 during growth in the cecum of germ-free C57 BL/6 IL-10 knockout mice to C. jejuni NCTC11168 during growth in Bolton broth.

2011-04-14 | GSE28578 | GEO

Transcriptional profile in macrolide susceptible and resistant Campylobacter jejuni

Project description:Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain. C. jejuni is prevalent in chickens, a natural reservoir for this pathogenic organism. Due to the importance of macrolide antibiotics in clinical therapy of human campylobacteriosis, development of macrolide resistance in Campylobacter has become a concern for public health.To facilitate understanding the molecular basis associated with the fitness difference between Erys and Eryr Campylobacter, we compared the transcriptomes between ATCC 700819 and its isogenic Eryr transformant T.L.101 using DNA microarray.

2011-11-02 | GSE33330 | GEO

Campylobacter jejuni murine adaptation: unadapted v. adapted

Project description:Expression arrays comparing Campylobacter jejuni 11168 before and after serial passage in C57 BL/6 IL-10 deficient mice. Gene expression was compared during exponential growth in Bolton broth.

2009-08-08 | GSE17562 | GEO

Defining stationary phase transcriptomes of Campylobacter jejuni cell morphotypes

Project description:Filamentation, the transition from rod to filamentous cell morphology, has been identified as a response to stressful conditions in many bacterial species. Campylobacter jejuni exhibits filamentation upon entry to stationary phase. This study examines the cell mophotype specific transciptomes (RNAseq) of C. jejuni.

2018-07-07 | GSE116729 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data