The value of twinned pollinator-pollen metabarcoding: bumblebee pollination service is weakly partitioned within a UK grassland community
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB58796 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR10760434.fastq.gz | Fastqsanger.gz | |||
| ERR10760435.fastq.gz | Fastqsanger.gz | |||
| ERR10760436.fastq.gz | Fastqsanger.gz | |||
| ERR10760437.fastq.gz | Fastqsanger.gz | |||
| ERR10760438.fastq.gz | Fastqsanger.gz |
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Project description:Predicting ecological impact of declining bumblebee (Bombus) populations requires better understanding of interactions between pollinator partitioning of floral resources and plant partitioning of pollinator resources. Here, we combine Cytochrome Oxidase 1 (CO1) barcoding for bumblebee identification and rbcL metabarcoding of pollen carried by bees in three species-rich UK pastures. CO1 barcoding assigned 272 bees to eight species, with 33 individuals belonging to the cryptic Bombus lucorum complex (16 B. lucorum and 17 B. cryptarum). Seasonal bias in capture rates varied by species, with B. pratorum found exclusively in June/July and B. pascuorum more abundant in August. Pollen metabarcoding coupled with PERMANOVA and NMDS analyses revealed all bees carried several local pollen species and evidence of pollen resource partitioning between some species pairings, with Bombus pratorum carrying the most divergent pollen load. There was no evidence of resource partitioning between the two cryptic species present, but significantly divergent capture rates concorded with previous suggestions of separation on the basis of foraging behaviour being shaped by local/temporal differences in climatic conditions. Considering the bee carriage profile of pollen species revealed no significant difference between the nine most widely carried plant species. However, there was a sharp, tipping point change in community pollen carriage across all three sites that occurred during the transition between late July and early August. This transition resulted in a strong divergence in community pollen carriage between the two seasonal periods in both years. We conclude that the combined use of pollen and bee barcoding offers several benefits for further study of plant-pollinator interactions at the landscape scale.
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Project description:Fungicides are used in agriculture to protect crops from various fungal diseases. However, they may modulate the plants metabolism. Moreover, fungicides can accumulate in the environment and may cause toxic effects on non-target organisms such as nectar microbes and pollinators. Nectar microbes contribute to the volatile profile of flowers and can influence pollinators behaviour. Thus, fungicide treatment could potentially affect the pollination. In this study, we investigated the influence of fungicide treatment on floral attributes as well as the behavioural impact on bumblebees. In separate experiments, we used one or both strawberry cultivars (Fragaria × ananassa var. Darselect and Malwina), which were either kept untreated (control) or treated with either Cuprozin® progress or SWITCH® fungicide. We analysed various flower traits including volatiles, pollen weight, pollen protein, and the attraction of bumblebees towards the flowers in the greenhouse. Additionally, we analysed the viability of pollen and pollen live-to-dead ratio, as well as the composition of nectar fungi in the field. A treatment with Cuprozin® progress led to a lower emission of floral volatiles and a slightly lower pollen protein content. This had no impact on the visit latency of bumblebees but on the overall visit frequency of these flowers. The treatment with the fungicide SWITCH® resulted in a higher emission of floral volatiles as well as a delayed first visit by bumblebees. Furthermore, flowers of control plants were visited more often than those treated with the two fungicides. Plant-pollinator interactions are highly complex, with many contributing factors. Fungicides can have an impact on the pollen quality and pollinator attraction, potentially leading to an altered pollen dispersal by pollinators and a change in fruit quality.
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Project description:DNA metabarcoding reveals a constancy in the foraging strategy of bumblebees in grasslands with high plant diversity after strong reduction of colony’s workforce.
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Project description:Monodelphis domestica MODO-UK, Monodelphis domestica HLA class I histocompatibility antigen, A-69 alpha chain-like (MODO-UK), mRNA. [Source:RefSeq mRNA;Acc:NM_001246247], is expressed in 2 baseline experiment(s);
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Project description:Bumblebees (Hymenoptera: Apidae) are important pollinating insects that play pivotal roles in crop production and natural ecosystem services. To achieve a comprehensive profile of accessible chromatin regions and provide clues for all possible regulatory elements in the bumblebee genome, we did ATAC-seq for Bombus terrestris samples derived from its four developmental stages: egg, larva, pupa, and adult, respectively. The sequencing reads of ATAC-seq were mapped to B. terrestris reference genome, and its accessible chromatin regions were identified and characterized using bioinformatic methods. Our study will provide important resources not only for uncovering regulatory elements in the bumblebee genome, but also for expanding our understanding of bumblebee biology.
2020-10-02 | GSE151858 | GEO
Project description:Bees often focus their foraging effort on a few or even a single flower species, even if other equally rewarding flower species are present. Although this phenomenon-called flower constancy-has been widely documented during single foraging trips, it is largely unknown if the behavior persists over longer time periods, especially under field conditions with large temporal variations of resources. We studied the pollen diet of individuals from nine different Bombus terrestris colonies for up to 6 weeks, to investigate flower constancy and pollen diversity of individuals and colonies, and how these change over time. We expected high degrees of flower constancy and foraging consistency over time, based on foraging theory and previous studies. Instead, we found that only 23% of the pollen foraging trips were flower constant. The fraction of constant pollen samples did not change over the study period, although repeatedly sampled individuals that were flower constant once often showed different preferences at other sampling occasions. The similarity of pollen composition in samples collected by the same individuals at different occasions dropped with time. This suggests that the flower preferences change in response to shifting floral resources. The average diversity of pollen from single foraging trips was around 2.5 pollen types, while the colony-level pollen diversity was about three times higher. How rapidly preferences change in response to shifting resources, and if this differs between and within bee species depending on factors such as size, should be the focus of future research.
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