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ICLIP Analysis of WT, phospho-defective, and phospho-mimicking mutants of mouse Nucleolin in iKras PDAC cells

ABSTRACT: iCLIP Analysis of WT, phospho-defective, and phospho-mimicking mutants of mouse Nucleolin in iKras PDAC cells

PROVIDER: PRJEB58806 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10755067.fastq.gz	Fastqsanger.gz
	ERR10755068.fastq.gz	Fastqsanger.gz
	ERR10755069.fastq.gz	Fastqsanger.gz
	ERR10755070.fastq.gz	Fastqsanger.gz
	ERR10755071.fastq.gz	Fastqsanger.gz

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iCLIP Analysis of WT, phospho-defective, and phospho-mimicking mutants of mouse Nucleolin in iKras PDAC cells

Project description:This experiment aimed to identify Nucleolin (Ncl) binding sites on a transcriptome-wide scale, by performing iCLIP in mouse iKras PDAC cells (Ying et al., 2012) transiently transfected with a mammalian expression construct encoding a wild-type (WT) myc-Ncl, a phospho-defective mutant in which S28, S34, S40, and S41 residues were mutated to A, or a phospho-mimicking mutant with these residues mutated to D. Cells transfected with an empty vector (myc-pRK5-DEST) were used as controls in the experiment.

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RIP-seq reveals the interactome of nucleolin in activated CD4+ T cells

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