Project description:The ability to rapidly respond to changes in temperature is critical for insects and other ectotherms living in variable environments. In a physiological process termed rapid cold-hardening (RCH), exposure to non-lethal low temperature allows many insects to significantly increase their cold tolerance in a matter of minutes to hours. Additionally, there are rapid changes in gene expression and cell physiology during recovery from cold injury, and we hypothesize that RCH may modulate some of these processes during recovery. In this study, we used a cDNA microarray to examine the molecular mechanisms of RCH and cold shock (CS) recovery in the flesh fly, Sarcophaga bullata. With our custom 2-color array, we measured expression of ~15,000 ESTs during RCH and during recovery from cold shock. Surprisingly, no transcripts were upregulated during RCH, and likewise, RCH had a minimal effect on the transcript signature during recovery from cold shock. However, during recovery from cold shock, we observed differential expression of ~1,400 ESTs, including a number of heat shock proteins, cytoskeletal components, and genes from several cell signaling pathways. Several gene pathways correlated well with metabolomics data, indicating that coordinated changes in gene expression and metabolism contribute to recovery from cold shock.

2012-03-14 | GSE36483 | GEO

Transcriptomics of rapid cold-hardening and cold shock recovery in Sarcophaga bullata

2012-03-14 | E-GEOD-36483 | biostudies-arrayexpress

Small RNA-seq reveals miRNAs that may regulate pupal diapause in the flesh fly, Sarcophaga bullata

Project description:Small RNA-seq was used to identify miRNAs in pupal stage Sarcophaga bullata and to assess differences in the abundance of miRNAs in developing and diapause (i.e. dormant) pupae.

2017-06-01 | GSE94376 | GEO

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