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RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

ABSTRACT: RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

PROVIDER: PRJEB60014 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR10903428_1.fastq.gz	Fastqsanger.gz
	ERR10903428_2.fastq.gz	Fastqsanger.gz
	ERR10903429_1.fastq.gz	Fastqsanger.gz
	ERR10903429_2.fastq.gz	Fastqsanger.gz
	ERR10903430_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

Project description:In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. As Arabidopsis thaliana is an annual plant we were interested in the plant defense and possible priming reaction of the closest biannual/perennial relative Arabidopsis lyrata. The main aim of the experiment was to compare the effect induced by insect egg deposition of Pieris brassicae between A. thaliana and A. lyrata plants grown in parallel on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 7 week old vegetative plants For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected.

2024-12-18 | E-MTAB-12653 | biostudies-arrayexpress

RNA-Seq of Arabidopsis thaliana plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory against untreated controls in two different developmental phases (vegetative vs. reproductive)

Project description:Plants can cope with stress better if they experience a mild form of the stress before the actual \\"real\\" stress event. In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. The main aim of the experiment was to compare the priming effect induced by insect egg deposition of Pieris brassicae between vegetative and reproductive (first open flowers) Arabidopsis thaliana plants on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 6 week old vegetative plants and 10 week old reproductive plants were the first flowers were open. For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected. From reproductive plants flower buds were collected as well.

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