Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CRISPRi on DMPK promoter

ABSTRACT: Highly Specific DMPK-promoter targeted by CRISPRi reverses DM1-associated defects in patient muscle cells

PROVIDER: PRJEB60168 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR11055011_1.fastq.gz	Fastqsanger.gz
	ERR11055011_2.fastq.gz	Fastqsanger.gz
	ERR11055012_1.fastq.gz	Fastqsanger.gz
	ERR11055012_2.fastq.gz	Fastqsanger.gz
	ERR11055013_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 70

Similar Datasets

Bisulfite-seq: Analysis of CpG methylation at two CTCF binding sites flanking the CTG repeats of DMPK.

Project description:Bisulfite sequencing was performed on GM04604 (UN-B), GM06077 (DM1-A), and GM04648 (DM1-B) patient cells to determine the levels of CpG methylation present at two CTCF sites closely flanking the CTG repeats of DMPK.

2020-04-07 | GSE148177 | GEO

Dmpk

Project description:Rattus norvegicus Dmpk, DM1 protein kinase [Source:RGD Symbol;Acc:1309825], is expressed in 4 baseline experiment(s);

| ENSRNOG00000015085 | ExpressionAtlas

Dmpk

Project description:Rattus norvegicus Dmpk, DM1 protein kinase [Source:RGD Symbol;Acc:1309825], is differentially expressed in 33 experiment(s);

| ENSRNOG00000015085 | ExpressionAtlas

DMPK

Project description:Homo sapiens DMPK, DM1 protein kinase [Source:HGNC Symbol;Acc:HGNC:2933], is expressed in 46 baseline experiment(s);

| ENSG00000104936 | ExpressionAtlas

DMPK

Project description:Homo sapiens DMPK, DM1 protein kinase [Source:HGNC Symbol;Acc:HGNC:2933], is differentially expressed in 147 experiment(s);

| ENSG00000104936 | ExpressionAtlas

DMPK

Project description:Macaca mulatta DMPK, DM1 protein kinase [Source:NCBI gene (formerly Entrezgene);Acc:715328], is differentially expressed in 1 experiment(s);

| ENSMMUG00000016457 | ExpressionAtlas

DMPK

Project description:Macaca mulatta DMPK, DM1 protein kinase [Source:NCBI gene (formerly Entrezgene);Acc:715328], is expressed in 3 baseline experiment(s);

| ENSMMUG00000016457 | ExpressionAtlas

Long-read sequencing for the assessment of therapeutic CRISPR/Cas9 strategy for the removal of aberrant DM1-causing CTG repeats in DMPK gene locus

Project description:The genetic cause of Myotonic Dystrophy Type 1 (DM1) is the expansion of unstable CTG-repeats in the 3´ untranslated region of the DMPK gene. Therapeutic gene editing for treatment of monogenic diseases could in principle remove definitively the disease-causing genetic defect. Here we describe the application of the CRISPR/Cas9 strategy to remove the CTG-expansion in the DMPK gene in myogenic cells derived from a DM1 patient that is heterozygous for the expansion. The efficacy of on-target genomic events was assessed by performing long-read DNA sequencing (Oxford Nanopore Technologies) after applying Cas9-mediated target enrichment of the Regions Of Interest (ROI), enabling the simultaneous analysis (a) of cuts by both sgRNAs resulting in the effective excision of the CTG expansion, (b) of cuts induced only by a single sgRNA, (c) of the potential occurrence of aberrant events such as inversions, and (d) the existence of non-edited sequences, within the same sample.

2025-02-26 | GSE279054 | GEO

Myotonic dystrophy type 1 (DM1) leads to altered mRNA expression in heart tissue

Project description:Myotonic dystrophy type 1 (DM1) is a dominantly inherited disease that affects multiple organ systems. Cardiac dysfunction is the second leading cause of death in DM1. We quantified gene expression in heart tissue from a heart-specific DM1 mouse model (EpA960/MCM) which inducibly expresses human DMPK exon 15 containing 960 CUG expanded repeats and that reproduced Celf1 up regulation. To assess if, in addition to splicing and miRNA defects, CUGexp RNA also perturbed the steady state mRNA levels of genes, we carried out a microarray study on wildtype E14, adult, MCM controls and DM1 mouse hearts. As anticipated we noted a large number of genes to be developmentally regulated in wildtype hearts, however, within 72h of induction of CUGexp RNA there appeared to be a coordinate adult-to-embryonic shift in steady state levels of many genes. We identified transcripts over-expressed or under-expressed in hearts of wildtype adult mice, wildtype embryonic day 14 (E14), and DM1 mice induced to express CUGexp RNA for 72h and 1wk, when compared to MCM controls. Multiple group comparison.

2014-01-24 | E-GEOD-48991 | biostudies-arrayexpress

Comparative analysis of Mbl attenuation and CUG non-coding repeats expression in Drosophila larval muscles

Project description:Myotonic dystrophy type 1 (DM1) is a neuro-muscular disorder caused by CTG triplet expansion in the 3-UTR of the DMPK gene. Mutated transcripts aggregate in muscle nuclei and sequester the MBNL1 splicing factor. To assess the involvement of Mbl sequestration on transcriptpion deregulation in DM1, we performed genome wide analyses of gene expression of DM1 Drosophila model in three different genetic contexts: Mef>mblRNAi, Mef>600CTG, Mef>960CTG vs. Mef>lacZ control line.

2013-04-01 | E-MTAB-1469 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data