Project description:Parascedosporium putredinis NO1 was grown for 4 days on six lignocellulosic substrates: Kraft Lignin (LI), Sugar Cane Bagasse (SC), Rice Straw (RS), Wheat Straw (WS), Wheat Bran (WB), and Empty Fruit Bunches from Palm Oil (EF). Proteins were harvested from the culture supernatant and from the insoluble fraction using a biotin-labelling approach to target the proteins bound to the lignocellulosic substrates.
Project description:Lignocellulose, the structural component of plant cells, is a major agricultural by-product and the most abundant terrestrial source of biopolymers on Earth. The complex and insoluble nature of lignocellulose limits its conversion into value-added commodities and, currently, efficient transformation requires expensive pre-treatments and high loadings of enzymes. Here we report on lignocellulolytic enzyme discovery from Parascedosporium putredinis NO1 during its deconstruction of wheat straw probing both the cultural supernatant and selecting for proteins bound to insoluble component of the growth culture.
Project description:Alistipes putredinis can alleviate DSS-induced colitis in mice, but the mechanism is unknown. The aim of this study was to examine the changes in gene expression in the intestinal tissue of mice with colitis following treatment with Alistipes putredinis by RNA sequencing.