Project description:To investigate dendritic cells-Leishmania interaction, the transcriptional profile of bone marrow-derived dendritic cells (BMDCs) infected with Leishmania infantum or of cells exposed to chemically inactivated parasites was assessed
Project description:We used PacBio data to identify more reliable transcripts from hESC, based on which we can estimate gene/transcript abundance better from Illumina data. PacBio long reads and Illumina short reads were generated from the same hESC cell line H1. PacBio reads were error-corrected by Illumina reads to identify transcripts. rSeq is used to estimate gene/transcript abundance of the identified transcriptome.
Project description:This dataset provides a proteomic resource of non-specific protein interactions in Leishmania infantum identified by affinity purification coupled to mass spectrometry (AP-MS/MS). Protein extracts from promastigotes expressing Cas9/T7 RNA polymerase were subjected to immunoprecipitation using HA, myc, and His affinity systems, followed by LC–MS/MS analysis. Raw spectral data were processed using MaxQuant against the L. infantum reference proteome from UniProtKB. The dataset includes raw mass spectrometry files, peptide and protein identification tables, and label-free quantification (LFQ) data, all publicly available via the ProteomeXchange Consortium under the identifier PXD067464. After data filtering, a total of 566 unique proteins were identified across all conditions, including 60 proteins consistently detected in all three affinity systems, representing putative non-specific binders. Among these, metabolism-related proteins (30%) and ribosomal components (28%) were the most abundant functional classes. This dataset provides a valuable reference for background protein binding in AP-MS/MS experiments in Leishmania , supporting improved interpretation of interactome studies and benchmarking of affinity purification strategies.
Project description:Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39 Keywords: stage-specific comparison Leishmania infantum: Two-condition experiment, promastigote stage vs amastigote stage. Six biological replicates for each stage, independently grown and harvested. One replicate per array Leishmania major: Two-condition experiment, promastigote stage vs amastigote stage. Four biological replicates for each stage, independently grown and harvested. One replicate per array