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Transcript Profiling after a Genetically-induced Elevation of Trehalose 6-Phosphate Reveals a Plethora of Direct and Indirect Responses

ABSTRACT: Transcript Profiling after a Genetically-induced Elevation of Trehalose 6-Phosphate Reveals a Plethora of Direct and Indirect Responses

PROVIDER: PRJEB60868 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11139135_1.fastq.gz	Fastqsanger.gz
	ERR11139135_2.fastq.gz	Fastqsanger.gz
	ERR11139136_1.fastq.gz	Fastqsanger.gz
	ERR11139136_2.fastq.gz	Fastqsanger.gz
	ERR11139137_1.fastq.gz	Fastqsanger.gz

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Transcript Profiling after a Genetically-induced Elevation of Trehalose 6-Phosphate Reveals a Plethora of Direct and Indirect Responses

Project description:We aim at understanding the Tre6P-specific signalling responses by transiently inducing the expression of the bacterial TPS (otsA) protein at the beginning of the day, leading to an induced rise in Tre6P levels. Treated plants- 2% ethanol spray. non-treated - water spray. empty-vector control - alcR (all previously described in Martins et al. (2013) and in Figueroa et al. (2016).

2023-04-02 | E-MTAB-12783 | biostudies-arrayexpress

Stress profile elicited by trehalose-6-phosphate accumulation in Mycobacterium tuberculosis

Project description:The otsB2 gene encoding trehalose-6-phosphate phosphatase is essential for in vitro growth of Mycobacterium tuberculosis and required to establish an acute infection in mice. Essentiality of otsB2 is due to direct or indirect toxic effects associated with the substrate trehalose-6-phosphate that accumulates when OtsB2 gene expression is impaired. In order to gain insight into the molecular basis of trehalose-6-phosphate mediated toxic effects, whole genome transcriptome profiling was done using RNA-seq. A conditional otsB2 mutant of Mycobacterium tuberculosis was generated by inserting an anhydrotetracycline-inducible promoter cassette upstream of the otsB2 start codon,and the transcriptome profile of a fully induced mutant resembling the wildtype phenotype was compared to that of a partially silenced mutant under conditions where 30% residual growth relative to the fully induced mutant was observed.

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Project description:In the present study, we investigated the consequences of trehalose intake on brain metabolism in mice drinking for 0, 1, and 10 days. Microarray analyses were performed to identify the molecular targets involved in the brain metabolism of trehalose intake.

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Highly concentrated trehalose induces prohealing senescence-like state in fibroblasts via CDKN1A/p21

Project description:Trehalose is the nonreducing disaccharide of glucose, evolutionarily conserved in invertebrates. The living skin equivalent (LSE) is an organotypic coculture containing keratinocytes cultivated on fibroblast-populated dermal substitutes. We demonstrated that human primary fibroblasts treated with highly concentrated trehalose promote significantly extensive spread of the epidermal layer of LSE without any deleterious effects. The RNA-seq analysis of trehalose-treated 2D and 3D fibroblasts at early time points revealed the involvement of the CDKN1A pathway, the knockdown of which significantly suppressed the upregulation of DPT, ANGPT2, VEGFA, EREG, and FGF2. The trehalose-treated fibroblasts were positive for senescence-associated β-galactosidase. Finally, transplantation of the dermal substitute with trehalose-treated fibroblasts accelerated wound closure and increased capillary formation significantly in the experimental mouse wounds in vivo, which was canceled by the CDKN1A knockdown. These data indicate that high-concentration trehalose can induce the senescence-like state in fibroblasts via CDKN1A/p21, which may be therapeutically useful for optimal wound repair.

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Direct and indirect responses of the Arabidopsis transcriptome to an induced increase in trehalose 6-phosphate.

Project description:Trehalose 6-phosphate (Tre6P) is an essential signal metabolite that regulates the level of sucrose, linking growth and development to the metabolic status. We hypothesized that Tre6P plays a role in mediating the regulation of gene expression by sucrose. To test this, we performed transcriptomic profiling on Arabidopsis (Arabidopsis thaliana) plants that expressed a bacterial TREHALOSE 6-PHOSPHATE SYNTHASE (TPS) under the control of an ethanol-inducible promoter. Induction led to a 4-fold rise in Tre6P levels, a concomitant decrease in sucrose, significant changes (FDR ≤ 0.05) of over 13,000 transcripts, and 2-fold or larger changes of over 5,000 transcripts. Comparison with nine published responses to sugar availability allowed some of these changes to be linked to the rise in Tre6P, while others were probably due to lower sucrose or other indirect effects. Changes linked to Tre6P included repression of photosynthesis-related gene expression and induction of many growth-related processes including ribosome biogenesis. About 500 starvation-related genes are known to be induced by SUCROSE-NON-FERMENTING-1-RELATED KINASE 1 (SnRK1). They were largely repressed by Tre6P in a manner consistent with SnRK1 inhibition by Tre6P. SnRK1 also represses many genes that are involved in biosynthesis and growth. These responded to Tre6P in a more complex manner, pointing toward Tre6P interacting with other C-signaling pathways. Additionally, elevated Tre6P modified the expression of genes encoding regulatory subunits of the SnRK1 complex and TPS class II and FCS-LIKE ZINC FINGER proteins that are thought to modulate SnRK1 function and genes involved in circadian, TARGET OF RAPAMYCIN, light, abscisic acid, and other hormone signaling.

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