Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tomato leaf stem and leaf blade type VI trichome head mRNA-seq

ABSTRACT: Tomato leaf stem and leaf blade type VI trichome head mRNA-seq

PROVIDER: PRJEB61150 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR11196420_1.fastq.gz	Fastqsanger.gz
	ERR11196420_2.fastq.gz	Fastqsanger.gz
	ERR11196421_1.fastq.gz	Fastqsanger.gz
	ERR11196421_2.fastq.gz	Fastqsanger.gz
	ERR11196422_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Different gene expression in the tomato trichome and leaf

Project description:We report the transcriptome difference between glandular cells of type VI trichome and leaf cells of trichome shaved leaf.

2021-07-26 | GSE117766 | GEO

Gene expression in the tomato trichome collected from af mutant and its complemented lines

Project description:We report the effect of chalcone isomerase deficiency on gene expression of glandular cells of type VI trichome in tomato leaves.

2021-07-27 | GSE118065 | GEO

Proteomics of Tomato Trichome Metabolism

Project description:Glandular trichomes (GT) are specialized cell factories that have the capacity to produce large amounts of metabolites which can amount to over 10% of the leaf dry weight. The specific expression of secondary metabolite pathways in glandular trichomes has facilitated their elucidation. However, little is known about the connection between central carbon and specialized metabolism in these cells. To address this question, we used the type VI glandular trichomes from a cultivated (Solanum lycopersicum LA4024) and a wild tomato accession (Solanum habrochaites LA1777) as a model. Our study is based on metabolomics, transcriptomics, proteomics and 13C-labeling datasets of trichome and leaves samples. This comparative analysis allowed us to identify specific features of trichomes in comparison to leaves. Tomato type VI trichomes are photosynthetic but acquire their carbon essentially from the leaves in the form of sucrose. Relatively high expression levels of photosystem genes and comparatively low expression of Calvin-cycle and photorespiration genes indicate that photosynthesis is used primarily to produce energy and reducing power to drive the high metabolic activity. High levels of polyunsaturated fatty acids, oxylipins, and of oxidized glutathione and high transcript and protein levels of associated enzymes further indicate a distinct capacity of GT to cope with oxidative stress. Enzymes which are involved in the supply of key precursors for the production of terpenoids both from the cytosol and in the chloroplasts are markedly increased. Finally, some enzymes that are typically associated with C4-photosynthesis are highly expressed in trichomes, suggesting that CO2 recycling processes may increase the efficiency of C usage in these cells. Based on this information we propose a model on how the glandular trichomes achieve high metabolic efficiency.

2017-07-04 | PXD003154 | Pride

Transcription profiling of Arabidopsis ecotype Columbia leaf tissue processed for trichome isolation

Project description:Transcriptional profiling of leaf tissue processed for trichome isolation.

2009-02-04 | E-MEXP-2014 | biostudies-arrayexpress

Arabidopsis thaliana trichome proteome

Project description:Trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and cell wall biogenesis. In this context, the exposed position, extraordinary size and characteristic morphology of trichomes featured particularly the exploration of trichome mutant phenotypes. However, trichome-specific biochemical or -omics analyses require a proper separation of trichomes from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions. To improve trichome-leaf separation, we revised a previously proposed method for isolating A. thaliana trichomes by optimizing the mechanical and biochemical specifications for trichome release. Furthermore, we introduced a density gradient centrifugation step to remove residual plant debris. We found that prolonged, yet mild seedling agitation increases the overall trichome yield by about 62% compared to the original protocol. We noticed that subsequent density gradient centrifugation further visually enhances trichome purity, which could be advantageous for downstream analyses. Histochemical and biochemical investigation of trichome cell wall composition indicated that gentle agitation during trichome release largely preserves trichome integrity. We used enriched and purified trichomes for proteomic analysis and present a reference data set of trichome-resident and -enriched proteins.

2022-01-13 | PXD028808 | Pride

Transcription profiling of Arabidopsis leaf pavement, trichome initial cells and mature trichomes

Project description:Comparison of A. thaliana leaf pavement, trichome initial cells and mature trichomes

2007-11-14 | E-MEXP-1038 | biostudies-arrayexpress

Different gene expression in the tomato trichome and leaf

Project description:Different gene expression in the tomato trichome and leaf

| PRJNA483134 | ENA

Wild tomato trichome transcriptome

Project description:Stem trichome mRNA-seq analysis of wild tomato relatives (Solanum section Lycopersicon)

| PRJEB44371 | ENA

Inducible expression of trichome regulators, GL1 and GL3

Project description:The development of trichomes (leaf hairs) from pluripotent epidermal cells in Arabidopsis provides a powerful system to investigate the regulatory motifs involved in plant cell differentiation. Genetic studies have revealed that a bHLH transcription factor, GL3, activates downstream genes required for trichome initiation by interacting with a R2R3-MYB protein, GL1. In order to investigate genome-wide regulatory functions of GL1 and GL3, we performed genome-wide expression analyses using GR inducible systems of GL1 and GL3.

2009-03-02 | GSE12551 | GEO

BC1F3 (S. hab PI127826 x S. lyc) mRNA-seq of type VI trichome heads

Project description:BC1F3 (S. hab PI127826 x S. lyc) mRNA-seq of type VI trichome heads

| PRJEB61104 | ENA