Project description:DNA replication is sensitive to damage in the template. To bypass lesions and complete replication, cells activate recombination-mediated (error-free) and translesion synthesis-mediated (error-prone) DNA damage tolerance pathways. Crucial for error-free DNA damage tolerance is template switching, which depends on the formation and resolution of damage-bypass intermediates consisting of sister chromatid junctions. Here we show that a chromatin architectural pathway involving the high mobility group box protein Hmo1 channels replication-associated lesions into the error-free DNA damage tolerance pathway mediated by Rad5 and PCNA polyubiquitylation, while preventing mutagenic bypass and toxic recombination. In the process of template switching, Hmo1 also promotes sister chromatid junction formation predominantly during replication. Its C-terminal tail, implicated in chromatin bending, facilitates the formation of catenations/hemicatenations and mediates the roles of Hmo1 in DNA damage tolerance pathway choice and sister chromatid junction formation. Together, the results suggest that replication-associated topological changes involving the molecular DNA bender, Hmo1, set the stage for dedicated repair reactions that limit errors during replication and impact on genome stability.

2014-01-28 | GSE46260 | GEO

Ribosome profiling reveals a high ribosomal occupancy and 5′ bias on mRNAs coding for aggregating proteins in HEK293 cells

Project description:Ribosome profiling of HEK293 control libraries used for the manuscript C1/C2 Ribo-seq GEO upload set. Files are staged in the dedicated GEO FTP subfolder RiboSeq.

2026-07-28 | GSE339435 | GEO

Error-free and error-prone DNA repair gene expression through reprogramming and passage in human iPS cells

Project description:Error-free and error-prone DNA repair gene expression through reprogramming and passage in human iPS cells

| PRJNA555421 | ENA

Single-Molecule Mechanical Analysis of Strand Invasion in Human Telomere DNA.

Project description: Not available

| S-EPMC9352315 | biostudies-literature

Mimicking damaged DNA with a small molecule inhibitor of human UNG2.

Project description: Not available

| S-EPMC1635321 | biostudies-literature

Single-molecule DREEM imaging reveals DNA wrapping around human mitochondrial single-stranded DNA binding protein.

Project description: Not available