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Establishment in Culture of Mouse Expanded Potential Stem Cells

ABSTRACT: Establishment in Culture of Mouse Expanded Potential Stem Cells

PROVIDER: PRJEB6168 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1013130.fastq.gz	Fastqsanger.gz
	ERR1013125.fastq.gz	Fastqsanger.gz
	ERR1013126.fastq.gz	Fastqsanger.gz
	ERR1013127.fastq.gz	Fastqsanger.gz
	ERR1013128.fastq.gz	Fastqsanger.gz

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Establishment of bovine expanded potential stem cells

Project description:Establishment of bovine expanded potential stem cells

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Establishment in Culture of Mouse Expanded Potential Stem Cells

Project description:The zygote and blastomeres of a cleavage stage mouse embryo have the capacity to differentiate to all lineages in the embryo proper and extraembryonic tissues and are considered totipotent. Mouse ESCs can differentiate to embryonic lineages but have restricted potential to trophoblasts. We report here that cultures of a new type of stem cells with expanded potential (EPSCs) can be established from in vitro cultured mouse preimplantation embryos and individual blastomeres, from directly converting mouse ESCs, or from genetically reprogramming somatic cells. EPSCs differentiate to trophoblasts in vitro, and a single EPSC contributes in chimeras to both extraembryonic lineages including the placenta trophoblasts and the embryo proper. Molecular analyses reveal that EPSCs express core pluripotency factors similar to ESCs, but have enriched signature of preimplantation blastomeres. The data described here suggest that similar cell lines from other mammalian species might also be established in culture and stably maintained.

2014-08-27 | E-ERAD-285 | biostudies-arrayexpress

Establishment of bovine expanded potential stem cells

Project description:Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eightcell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.

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Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

Project description:Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

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Project description:Despite intensive efforts, establishing porcine embryonic stem cells have been challenging. We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the activity of critical molecular pathways that predisposes lineage differentiation in the mouse preimplantation embryo. EPSCs had enriched molecular signatures of blastomeres and possessed the developmental potency to all embryonic and extraembryonic cell lineages. In this study, we report the derivation of porcine EPSC (pEPSC) lines either directly from preimplantation embryos or by reprogramming fetal fibroblasts. Under similar culture conditions, human ESCs and iPSCs can be converted, or somatic cells are directly reprogrammed, to EPSCs (hEPSCs) that display the molecular and functional attributes reminiscent of pEPSCs. Here, we performed ChIP-seq experiments of H3K4me3 and H3K27me3 to characterise the epigenetic signatures of the EPSCs.

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Project description:We described a new culture system that can expand the developmental potential of pluripotent stem cells to contribute efficiently to extraembryonic lineage development. To compare the epigenetic landscape of expanded potential stem cells (EPSCs) and standard embryonic stem cells, we performed ChIP-seq study on multiple histone modifications to establish the epigenetic landscape of EPSCs.

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