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Sequencing of murine corticotrophs from male and female POMC-GFP mice.

ABSTRACT: Sequencing of murine corticotrophs from male and female POMC-GFP mice.

PROVIDER: PRJEB61807 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR11282025_1.fastq.gz	Fastqsanger.gz
	ERR11282025_2.fastq.gz	Fastqsanger.gz
	ERR11282026_1.fastq.gz	Fastqsanger.gz
	ERR11282026_2.fastq.gz	Fastqsanger.gz
	ERR11282027_1.fastq.gz	Fastqsanger.gz

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Sequencing of murine corticotrophs from male and female POMC-GFP mice.

Project description:Stress-related illness represents a major burden on health and society. Understanding the molecular mechanisms underlying stress responses is an important step to understand these diseases, and design treatments. Sexual dimorphism in stress responses have been extensively reported, with depression and most anxiety disorders being more prevalent in women. Corticotrophs of the anterior pituitary play a central role in the generation of hormonal stress responses, by secreting the stress hormone ACTH in response to stressful stimuli. However, their role in contributing to sexually dimorphic responses of the HPA axis is very poorly understood. We performed bulk RNA-sequencing of FACS-sorted corticotrophs from male and female POMC-GFP mice to determine the molecular determinants of sexual dimorphic traits in the activity of these cells, revealing extensive differential gene expression at the transcriptional level. This includes more than 71 mRNAs encoding ion channel subunits, which could be involved in the generation of the sexual dimorphism in their electrical activity.

2023-05-03 | E-MTAB-12885 | biostudies-arrayexpress

Sequencing of murine corticotrophs from male POMC-GFP mice subjected to chronic restraint stress

Project description:Sequencing of murine corticotrophs from male POMC-GFP mice subjected to chronic restraint stress

| PRJEB71414 | ENA

Sequencing of murine corticotrophs from male POMC-GFP mice subjected to chronic restraint stress

Project description:Stress-related illness represents a major burden on health and society. Understanding the molecular mechanisms underlying stress responses is an important step to understanding these diseases and designing treatments. Corticotrophs of the anterior pituitary play a central role in the generation of hormonal stress responses, by secreting the stress hormone ACTH in response to stressful stimuli. However, it is poorly understood how they change in response to chronic stress, and whether they return to their original state after the stress has ended. We performed bulk RNA-sequencing of FACS-sorted corticotrophs from male POMC-GFP mice before or after exposure to chronic stress, and after 4 or 12 weeks of recovery, to determine the effect of chronic stress (and of recovery) on corticotrophs transcriptome. This revealed extensive differential gene expression, especially during the recovery period. N.B. Some of the control samples in this dataset (CM1, 2, and 3) are shared with the related experiment, E-MTAB-12885.

2024-10-30 | E-MTAB-13648 | biostudies-arrayexpress

male and female poplar leaves phosphoproteome

Project description:Nitrogen is a key factor impacting plant physiological processes and protein abundance. Although many proteins were changed substantially in poplar under N deficiency, the post-translational modifications in male and female poplars are still unclear. Therefore, we selected male and female poplars and analysed the changes of protein phosphorylation in response to N-deficient conditions.

2020-01-31 | PXD016397 | Pride

Expression analysis of Plasmodium falciparum male and female gametocytes

Project description:Investigation of whole genome gene expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

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Transcriptome analysis of immature and mature male and female gametocytes of the malaria parasite Plasmodium falciparum

Project description:In the human host, Plasmodium falciparum parasites propagate asexually in erythrocytes causing the symptoms of malaria. However, in every asexual cycle, a small proportion of the parasites commits to sexual differentiation, which is critical for transmission. During a period of about 10 days, the sexually committed parasites either develop into male or female gametocytes. In this study, gametocytes carrying an endogenously GFP tagged version of the female specifically expressed ABCG2 gene were FACS sorted to separate male (GFP low) and female (GFP high) gametocyte populations at different days of development. RNA was subsequently extracted from these populations as well as from asexual parasites as controls and subjected to directional RNAseq. These data reveal the transcriptional differences between asexual parasite stages and developing gametocytes, but also between male and female gametocytes as well as for each sex during the course of differentiation. In particular, male gametocytes undergo distinct transcriptional transitions when comparing the transcriptome between day 4, 6 and 10 of gametocytogenesis. In contrast, female gametocyte transcriptomes are more static during the course of gametocytogenesis and only show minimal variation. This is the first study to longitudinally monitor gene expression in developing male and female gametocytes

2021-07-31 | GSE180985 | GEO

Real-time quantitative PCR analysis of Vegf knockdown on expression of extracellular matrix and adhension molecules in male and female primary murine osteoblasts

Project description:Primary murine osteoblasts, isolated from the long bones of 4-day old male and female Vegffl/fl mice, were cultured prior to transduction with adenovirus-Cre-GFP at an multiplicity of infection of 100, to knockdown Vegf expression (OBVEGFKO) in vitro for six days. Control cells were transduced with an adenovirus expressing GFP (WT). Following this, cells were stepped down in low serum media containing 1% FBS for 24 hours, after which the conditioned media was collected for knockdown validation using an VEGF ELISA. We used the Qiagen Extracellular Matrix and Adhension Molecule RT2 Profiler PCR Array to assay the sexually dimorphic effects of Vegf on the expression of genes associated with bone matrix formation and mineralisation, remodelling and angiogenesis.

2021-02-28 | GSE164735 | GEO

ChIP-Seq analysis of day 6 male and female gametocytes of the malaria parasite Plasmodium falciparum

Project description:Plasmodium falciparum parasites alternate between two different obligate hosts during their life cycle: humans and Anopheles mosquitoes. During the blood stage in the human host they proliferate asexually inside erythrocytes. A small proportion of parasites develops into male and female gametocytes, which enter the sexual part of the life cycle once taken up by a mosquito. The production of male and female gametocytes is therefore critical for malaria transmission. It has been shown that epigenetic processes play a role in this differentiation processes, however, the exact mechanisms remain unknown. To gain insight into these processes, we separated male and female gametocytes, based on the female specific expression of an endogenously GFP-tagged ABCG2 gene, using flow cytometry (male parasites as GFP low, female parasites as GFP high population). We subsequently performed ChIP-Seq for several histone variants and modifications (H2A.Z, H2A.Zac, H2B.Z, H3K4me3, H3R17me2, H3K27ac, H3K9me3) on day 6 of gametocytogenesis. Our study reveals a global remodelling of the chromatin landscape in gametocytes compared to asexual parasites, as well as sex specific differences.

2022-05-07 | GSE202214 | GEO

Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes

Project description:Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-30 | GSE77398 | GEO

The Purpose of This Study is to Determine the Frequency of Colorectal Cancer in Male and Female Endurance Athletes Between the Ages of 35 and 50

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