ABSTRACT: EMG produced TPA metagenomics assembly of PRJEB52748 data set (Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides).
Project description:In this study, we quantitated the disappearance of intact HMOs and characterized the glycan digestion products in the gut that are produced by the action of microbial enzymes on HMOs and glycoconjugates from breast milk. Oligosaccharides from fecal samples of exclusively breast-fed infants were extracted and profiled using nanoLC-MS. Intact HMOs were found in the fecal samples, additionally, other oligosaccharides were found corresponding to degraded HMOs and non-HMO based compounds. The latter compounds were fragments of N-glycans released through the cleavage of the linkage to the asparagine residue and through cleavage of the chitobiose core of the N-glycan.
Project description:With this study, we wanted to investigate degradation of human milk oligosaccharides and the subsequent cross-feeding interactions of a complex bacterial community that resembles the gut microbiota of pre-weaned vaginally-born breastfed infants.
2024-08-27 | PXD047785 | Pride
Project description:EMG produced TPA metagenomics assembly of PRJNA768833 data set (Metagenomic Sequencing of Rural Kenyan Fecal Samples).
| PRJEB63400 | ENA
Project description:EMG produced TPA metagenomics assembly of the PRJEB27005 data set (Gut metagenomes of rural populations in Cameroon).
Project description:To discover the regulatory role of human milk exosomes oligosaccharides, The full cDNA was extracted from human macrophages and then compared the expression profiles of them to discovery the key features regulated by human milk exosomes extracted oligosaccharides.
2021-10-06 | GSE163125 | GEO
Project description:EMG produced TPA metagenomics assembly of PRJEB30246 data set (Metagenomic_analysis_nasopharyngeal_colonisation).
| PRJEB75197 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA639280 data set (v).
| PRJEB72785 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA986291 data set (CForBio.metagenome).