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OmpR binding peaks identification by ChIP-seq analysis

ABSTRACT: Identification the binding peaks of Vibrio parahaemolyticus OmpR by ChIP-seq analysis to investigate the mechanisim of OmpR in molecular level.

PROVIDER: PRJEB63095 | ENA |

REPOSITORIES: ENA

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OmpR binding peaks identification by ChIP-seq analysis

Project description:Identification the binding peaks of Vibrio parahaemolyticus OmpR by ChIP-seq analysis to investigate the mechanisim of OmpR in molecular level.

| PRJEB63214 | ENA

From environmental adaptation to virulence: OmpR plays an important role in Vibrio vulnificus

Project description:Vibrio vulnificus (V. vulnificus) is an opportunistic human pathogen known for causing various illnesses such as gastroenteritis, skin and muscle necrosis, septic shock, and sepsis. This halophilic estuarine bacterium's growth and infection process involves adaptation to both the natural briny environments and the host. OmpR, a response regulator in the EnvZ/OmpR two-component regulatory system (TCS), is crucial for environmental adaptation and pathogenicity. This study focused on investigating the impact of OmpR in V. vulnificus by creating an ompR knockout strain (ΔompR). The ΔompR strain exhibited reduced tolerance to alkaline stress, shorter flagella, and decreased virulence in epithelial cell and mouse models compared to the wild-type (WT) V. vulnificus. RNAseq analysis revealed the downregulation of genes involved in metabolism, flagellum-dependent motility, and transcription factors in the ΔompR strain. OmpR was found to repress the expression of aphB in alkaline conditions, impacting the acid resistance system CadBA, while also positively regulating the transcription of various flagellar genes. These findings suggest that OmpR acts as a global regulator, orchestrating the expression of multiple genes in response to different environments and during host invasion.

2024-11-08 | GSE281430 | GEO

Identification of differentially expressed host microRNAs that respond to Vibrio parahaemolyticus infection in the pacific white shrimp Litopenaeus vannamei.

Project description:In order to gain a better understanding of the impact of Vibrio parahaemolyticus infection on genetic regulation of Litopenaeus vannamei,we performed a miRNA-seq analysis in the hepatopancreas of Litopenaeus vannamei challenged with Vibrio parahaemolyticus, using the Illumina HiSeq 2500 platform.

2020-06-18 | GSE107696 | GEO

ChIP-on-chip of OmpR binding in Salmonella enterica serovar Typhimurium strain SL1344 and E.scherichia coli strain CSH50 at pH 7 and pH 4.5 including analysis of OmpR binding in SL1344 in the absence and presence of novobiocin (25 μg/ml)

Project description:OmpR is a DNA binding protein belonging to the OmpR/EnvZ two component system. This system is known to sense changes in osmolarity in Escherichia coli. Recently, OmpR in Salmonella enterica serovar Typhimurium was found to be activated by acidic pH and DNA relaxation. In this study, ChIP-on-chip was employed to ascertain the genome-wide distribution of OmpR in Salmonella Typhimurium and Escherichia coli in acidic and neutral pH. In addition we investigated the affect of DNA relaxation on OmpR binding in Salmonella Typhimurium.

2014-03-27 | GSE49914 | GEO

Expression profiling of EvgSc and ompR mutants

Project description:In order to explore the transcriptional reprogramming in EvgSc, ompR and EvgSc/ompR mutants we have used whole genome microarray profiling. EvgSc, ompR and EvgSc/ompR mutants were developed. Each mutant was profiled in duplicate.

2015-05-20 | E-GEOD-56833 | biostudies-arrayexpress

Expression profiling of EvgSc and ompR mutants

Project description:In order to explore the transcriptional reprogramming in EvgSc, ompR and EvgSc/ompR mutants we have used whole genome microarray profiling.

2015-05-20 | GSE56833 | GEO

OmpR Arg-GlcNAcylation within Salmonella

Project description:Analysis of OmpR Arg-GlcNAcylation using bottom up proteomic analysis

2023-05-20 | PXD039412 | Pride

Transcriptome analysis of the Salmonella enterica OmpR regulon

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type and ompR mutant grown to mil-exponential phase in LB. The goal was to define the ompR-regulated genes.

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Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis

Project description:Background The osmotic regulator OmpR in Escherichia coli regulates differentially the expression of major porin proteins OmpF and OmpC. In Yersinia enterocolitica and Y. pseudotuberculosis, OmpR is required for both virulence and survival within macrophages. However, the phenotypic and regulatory roles of OmpR in Y. pestis are not yet fully understood. Results Y. pestis OmpR is involved in building resistance against phagocytosis and controls the adaptation to various stressful conditions met in macrophages. The ompR mutation likely did not affect the virulence of Y. pestis strain 201 that was a human-avirulent enzootic strain. The microarray-based comparative transcriptome analysis disclosed a set of 224 genes whose expressions were affected by the ompR mutation, indicating the global regulatory role of OmpR in Y. pestis. Real-time RT-PCR or lacZ fusion reporter assay further validated 16 OmpR-dependent genes, for which OmpR consensus-like sequences were found within their upstream DNA regions. ompC, F, X, and R were up-regulated dramatically with the increase of medium osmolarity, which was mediated by OmpR occupying the target promoter regions in a tandem manner. Conclusion OmpR contributes to the resistance against phagocytosis or survival within macrophages, which is conserved in the pathogenic yersiniae. Y. pestis OmpR regulates ompC, F, X, and R directly through OmpR-promoter DNA association. There is an inducible expressions of the pore-forming proteins OmpF, C, and X at high osmolarity in Y. pestis, in contrast to the reciprocal regulation of them in E. coli. The main difference is that ompF expression is not repressed at high osmolarity in Y. pestis, which is likely due to the absence of a promoter-distal OmpR-binding site for ompF.

2011-10-24 | GSE26601 | GEO

Relationship between sex and disease resistance of Penaeus vannamei

Project description:To investigate the relationship between the resistance of male and female Penaeus vannamei and their immunity， we collected hemocytes from shrimps stimulated by Vibrio parahaemolyticus.

2024-04-10 | GSE253240 | GEO

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