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ABSTRACT: EMG produced TPA metagenomics assembly of PRJNA769968 data set (Probiotic consumption relieved Parkinson's disease via modulating the gut microbiota and neuroactive compounds).

PROVIDER: PRJEB63522 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR16279910.fasta.gz	Fasta.gz
	SRR16279925.fasta.gz	Fasta.gz
	SRR16279915.fasta.gz	Fasta.gz
	SRR16279920.fasta.gz	Fasta.gz

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Project description:We used Affymetrix microarrays to investigate gene expression changes in the liver of wild-type C57BL-6 mice exposed to a high-fat diet that might have been caused by the oral consumption of the probiotic B. pseudocatenulatum CECT 7765. The aim of this work was to determine whether the daily intake (by oral gavage) of the probiotic (P) B. pseudocatenulatum for seven weeks exerted any modulatory effects, at the level of gene expression, in the liver of C57BL-6 male mice exposed to a high-fat diet (HFD). Male mice were randomly assigned to four experimental groups (n= 5 animals per group) as follows: (1) control group, fed a standard diet (SD); (2) obese group, fed a high-fat diet (HFD); (3) a group that received the SD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (SD+P); and (d) an obese group that was fed the HFD and a daily dose of the probiotic (1M-CM-^W109 CFU B. pseudocatenulatum CECT 7765) (HFD+P). At the end of the experimental procedure total RNA was extracted from the liver to compare differential gene expression between the groups. Liver differential gene expression after 7 weeks of supplementation between: 1) the HFD group and the SD group (effects of the high-fat diet); 2) the HFD+P and the HFD (effects of the probiotic on the consumption of a high-fat diet) and 3) the SD+P group and the SD (direct effects of the probiotic on the liver of animals consuming a normal diet).

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Project description:EMG produced TPA metagenomics assembly of PRJNA834801 data set (Characterizing dysbiosis of the Parkinson's disease gut microbiome using shotgun metagenomics).

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