Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stability of Arabidopsis thaliana seeds under exposure of ultra-high static magnetic

ABSTRACT: Evaluation on DNA stability under exposure of ultra-high static magnetic fields up to 33 Tesla in dried Arabidopsis thaliana seeds

PROVIDER: PRJEB65433 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR12095800_1.fastq.gz	Fastqsanger.gz
	ERR12095800_2.fastq.gz	Fastqsanger.gz
	ERR12095900_1.fastq.gz	Fastqsanger.gz
	ERR12095900_2.fastq.gz	Fastqsanger.gz
	ERR12096000_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 472

Similar Datasets

Transcriptome profiles of differentiated mouse embryonic stem cells cultured in normal condition and static magnetic field condition by RNA-seq analysisanalysis

Project description:Embryonic stem cells (ESCs) have the ability to differentiate into cells of the three germ layers, and leukemia inhibitory factor (LIF) maintains the pluripotency and promotes the proliferation of ESCs. In the absence of LIF, ESCs spontaneously differentiate and form three-dimensional aggregates known as embryoid bodies (EBs). The differentiation of EBs mimics the process of embryonic development, that is, the differentiation of cells into the three embryonic germ layers (endoderm, mesoderm, and ectoderm), some of which differentiate into beating cardiomyocytes. Static magnetic fields have diverse effects on organisms, studies on the regulation of the differentiation of ESCs to cardiomyocytes by static magnetic fields are not sufficient. To better understand transcriptional landscape and signal transductions, we performed RNA-seq analysis of EBs cultured in two different conditions: conventional incubator, static magnetic field incubator.

2024-01-01 | GSE206413 | GEO

Effects of magnetite nanoparticles and static magnetic field on neural differentiation of pluripotent stem cells

Project description:Neural proliferation and differentiation fates of pluripotent stem cells are influenced by external natural forces. Despite the presence of biogenic magnetite nanoparticles in the central nervous system and constant exposure to Earth’s magnetic fields and other sources, there has been scant knowledge regarding the role of electromagnetic stimuli in neurogenesis. Moreover, the emerging application of electrical and magnetic stimulation to treat neurological disorders emphasizes the relevance of understanding the impact and mechanisms behind these stimuli. Here, the effects of magnetic nanoparticles (MNPs) contained in polymeric coatings and the static external magnetic field (EMF, 0.4 Tesla) were investigated on neural induction of murine embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) into induced dopaminergic neurons (iDA).

2021-12-17 | GSE190686 | GEO

The Effects of Static Magnetic Fields on Human Embryonic Cells

Project description:This study provides a framework describing how magnetic exposure is transduced from the most-plausible molecular-level ‘biosensor’ (lipid membranes) to cell-level responses that include differentiation toward neural lineages. In addition, SMF provided a stimulus that uncovered new relationships – that exist even in the absence of magnetic fields – between gangliosides, the time dependent regulation of IL-6 signaling by these glycolipids, and the fate of embryonic cells.

2010-01-18 | GSE14474 | GEO

Static Magnetic Fields Regulate T-Type Calcium Ion Channels and Mediate Mesenchymal Stem Cells Proliferation

Project description:The static magnetic fields (SMFs) impact on biological systems, induce a variety of biological responses, and have been applied to the clinical treatment of diseases. However, the underlying mechanisms remain largely unclear. In this report, by using human mesenchymal stem cells (MSCs) as a model, we investigated the biological effect of SMFs at a molecular and cellular level. We showed that SMF exposure promotes MSC proliferation and activates the expression of transcriptional factors such as FOS (Fos Proto-Oncogene, AP-1 Transcription Factor Subunit) and EGR1 (Early Growth Response 1). In addition, the expression of signal-transduction proteins p-ERK1/2 and p-JNK oscillate periodically with SMF exposure time. Furthermore, we found that the inhibition of the T-type calcium ion channels negates the biological effects of SMFs on MSCs. Together, we revealed that the SMFs regulate T-type calcium ion channels and mediate MSC proliferation via the MAPK signaling pathways.

2022-08-10 | GSE210518 | GEO

Next Generation Sequencing Facilities Quantitative Analysis of Wild type CD8+ T cells with 0.3T static magnetic field or not

Project description:Purpose:Next Generation Sequencing applying for compare the CD8+ T cells with 0.3T static magnetic field (SMF) or not transcriptome, included pathway-enrichment analysis and genes expression of interests Methods:CD8+ T cells were isolated from splenocytes in 8 week old wild type mice through STEMCELL Technologies negative selection kit. FACS sort CD8+ T cells after 3 days with CD3/CD28 costimulation at 0.3T static magnetic field or not. RNA was extracted, purified and checked for integrity using an Agilent Bioanalyzer 2100. Libraries were generated for sequencing using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. Libraries were sequenced using an Illumina HiSeq X Ten sequencer. Resluts: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to further analyse Conclusions:Our study represents the detailed analysis of CD8+ T cells with 0.3T static magnetic field or not transcriptomes

2020-09-08 | GSE113858 | GEO

Static Magnetic Fields Regulate T-Type Calcium Ion Channels and Mediate Mesenchymal Stem Cells Proliferation

Project description:Static Magnetic Fields Regulate T-Type Calcium Ion Channels and Mediate Mesenchymal Stem Cells Proliferation

| PRJNA866081 | ENA

Effect of extremely low frequency weak magnetic fields

Project description:We used AML12 cells exposured with pulsed extremely low frequency weak magnetic fields for 4 msec at increasing frequency of 1, 2, 3, 4, 5, 6, 7, and 8 Hz for 1 sec each. The 1-to-8 Hz pulses over 8 sec were repeated indefinitely. Peak magnetic intensity was 100 mG. AML12 cells were incubated for 1 h under extremely low frequency weak magnetic fields.

2022-05-05 | GSE166811 | GEO

Transcription profiling of human embryonic cells to investigate the effects of standing magnetic fields

Project description:This study provides a framework describing how magnetic exposure is transduced from the most-plausible molecular-level âbiosensorâ (lipid membranes) to cell-level responses that include differentiation toward neural lineages. In addition, SMF provided a stimulus that uncovered new relationships â that exist even in the absence of magnetic fields â between gangliosides, the time dependent regulation of IL-6 signaling by these glycolipids, and the fate of embryonic cells. Experiment Overall Design: Subconfluent (70%-80%) undifferentiated LVECs (passage 11) were resuspended at 500k cells in 10 ml culture medium (day 0) and cultured in the presence or absence of static magnetic field. Four conditions were investigated: A) Control (without exposure); B) One day exposure (Exposed on day 6 without recovery); C) Continuous exposure (day 2 to day 5) followed by one day recovery; D) Continuous exposure (day 2 to day 6) without recovery. Cells are harvested on day 7. mRNA was isolated from the cells and microarray analysis was done using the Affymetric Human Genome U133 2.0 Plus Chip.

2010-01-23 | E-GEOD-14474 | biostudies-arrayexpress

Effects of magnetite nanoparticles and static magnetic field on neural differentiation of pluripotent stem cells

Project description:Effects of magnetite nanoparticles and static magnetic field on neural differentiation of pluripotent stem cells

| PRJNA787959 | ENA

Effect of extremely low frequency weak magnetic fields

Project description:Effect of extremely low frequency weak magnetic fields

| PRJNA702060 | ENA