Project description:To determine the pleiotropic effect of the ABC transporter gene (Atpdr2) mutation, we performed the microarray analyses on the root tissues of Arabidopsis thaliana wild type (Col-0) and Atpdr2 mutant.
Project description:We have used the genetic resources of Arabidopsis thaliana to generate mutant lines that have reactivated TE expression. We used these lines with long-read Oxford Nanopore sequencing technology to capture Transposable Element (TE) mRNAs for TE transcript annotation.
Project description:Serendipita indica, a widely studied beneficial root fungal endophyte, promotes plant growth under saline conditions by reducing Na⁺ accumulation in host plants, including Arabidopsis thaliana. This reduction in Na⁺ levels likely contribute to salt detoxification, though the underlying mechanisms remain unclear. Previous studies have demonstrated that SOS1, a key Na+ transporter and major determinant of salt tolerance in plants, is not involved in this reduction. To explore whether other plant transporters might participate in Na+ reduction, we first characterized the full Arabidopsis transportome, with putative substrates and subcellular localizations, and performed a comprehensive transcriptomic analysis of the full set of transporter proteins. In this study, we investigated and ruled out the possible contribution of HKT1, another relevant Na+ transporter implicated in salt adaptation in Arabidopsis. By examining differentially expressed transporters under salt stress, we identified a subset of candidate genes potentially involved in Na⁺ transport. Among these, we evaluated the role of CNGC10 and CNGC13 using mutant lines under both S. indica- and non-colonized conditions. Interestingly, both transporters appeared to be involved in the endophyte-induced decrease in Na⁺ accumulation although, other, yet-unidentified transporters may also contribute to this phenomenon, warranting further functional investigation of the candidate genes highlighted in our analysis.
Project description:The wild-type and sdg8 mutant Arabidopsis thaliana Histones were extracted from 15-day-old Arabidopsis seedlings，Histone peptides were digested with trypsin for QTRAP 6500 mass spectrometry (MS) analysis.
