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Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis

ABSTRACT: Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis

PROVIDER: PRJEB65921 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12029859.fastq.gz	Fastqsanger.gz
	ERR12029860.fastq.gz	Fastqsanger.gz
	ERR12029861.fastq.gz	Fastqsanger.gz
	ERR12029862.fastq.gz	Fastqsanger.gz
	ERR12029863.fastq.gz	Fastqsanger.gz

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Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis

Project description:The experiment aimed to find how Campylobacter responds to oxidative stress using hydrogen peroxide. This was done by using previously made TraDIS libraries (https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02835-8) and putting them under oxidative stress. The strains used were C. jejuni 11168, C. coli 15-537360 and C. coli CCN182.

2023-09-14 | E-MTAB-13336 | biostudies-arrayexpress

Campylobacter jejuni genes contributing to acid adaptation

Project description:In order to cause disease, the food- and water-borne pathogen Campylobacter jejuni must face the extreme acidity of the host stomach as well as cope with pH fluctuations in the intestine. In the present study, C. jejuni NCTC 11168 was grown under mild acidic conditions mimicking those encountered in the intestine. The resulting transcriptional profiles revealed how this bacterium fine-tunes gene expression in response to acid stress. This adaptation involves differential expression of respiratory pathways, induction of genes for phosphate transport and repression of energy generation and intermediary metabolism genes. Keywords: acid shock; dose response; transcriptional response to 3 mild-acidic pH growth conditions (pH6.5, pH 6.0 and pH5.0)

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Campylobacter jejuni butyrate resopnse

Project description:Growth of Campylobacter jejuni in butyrate, including deletions of a TCS involved in sensing this response

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Campylobacter gene regulation in C. jejuni

Project description:The pathogenic bacterium Campylobacter jejuni is the leading cause of bacterial foodborne gastroenteritis worldwide yet it does not grow in the aerobic environment. The paralogues RrpA and RrpB which are members of MarR family of DNA binding proteins have been shown to be important for the survival of C. jejuni under aerobic and redox stress. We report that RrpA is a positive regulator of mdaB, encoding a flavin-dependent quinone reductase. MdaB confers protection to the cell from redox stress mediated by structurally diverse quinones. RrpB negatively regulates the expression of nfrA (Cj1555c), a flavin reductase. NfrA reduces riboflavin at a much higher rate than flavin mononucleotide (FMN),suggesting exogenous free flavins are the natural substrate. Enzymatic activity of MdaB and NfrA towards their substrates revealed both reductases preferred NADPH as an electron donor. DNA-binding and post translational modification analyses showed that the mechanism of RrpA and RrpB DNA binding is likely a cysteine-based redox switch. Complete genome sequences analysis indicated that MdaB is predominant in Campylobacter spp. and the related Helicobacter spp., whilst NfrA is more often found in C. jejuni strains. Quinones and flavins are antimicrobial redox cycling agents secreted by a wide range of cell-types that can form damaging superoxide by one-electron reactions. We propose that MdaB and NfrA production allows a two-electron reduction mechanism to the less toxic quinol forms. These enzymes thus aid the survival and persistence of C. jejuni in the face of toxic compounds from competing microbes.

2021-11-03 | PXD028910 | Pride

Diarrhoeal mechanisms of the Campylobacter jejuni enteritis

Project description:Campylobacter jejuni is the most prevalent cause of foodborne bacterial enteritis worldwide. This study aims at the characterisation of pathomechanisms and signalling in Campylobacter-induced diarrhoea in the human mucosa. During routine colonoscopy, biopsies were taken from patients suffering from campylobacteriosis. RNA-seq of colon biopsies was performed to describe Campylobacter jejuni-mediated effects. Mucosal mRNA profiles of acutely infected patients and healthy controls were generated by deep sequencing using Illumina HiSeq 2500. This data provide the basis for subsequent upstream regulator analysis.

2017-10-10 | GSE88710 | GEO

The Campylobacter jejuni transcriptional regulator CosR plays a role in the oxidative and aerobic stress response and is important for bacterial survival in vivo

Project description:Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment. C. jejuni must possess finely tuned regulatory systems to sense and adapt to external stimuli such as oxidative and aerobic (O2) stress. Re-annotation of the C. jejuni NCTC11168 genome sequence identified Cj1556 (originally annotated as a hypothetical protein) as a MarR family transcriptional regulator and further bioinformatic analysis indicated a role in regulating the oxidative stress response. A C. jejuni 11168H Cj1556 mutant exhibited increased sensitivity to oxidative and aerobic stress, decreased ability for intracellular survival in both Caco-2 human intestinal epithelial cells and J774A.1 mouse macrophages and a reduction in virulence in the Galleria mellonella infection model. Microarray analysis of gene expression changes in the Cj1556 mutant compared to the wild-type strain indicated negative autoregulation of Cj1556 expression and down-regulation of genes associated with oxidative and aerobic stress responses, such as katA, perR and hspR. cprS, which encodes a sensor kinase involved in the regulation of biofilm formation, was also up-regulated in the Cj1556 mutant and subsequent studies showed that the Cj1556 mutant had a reduced ability to form biofilms. Protein interaction studies confirmed the binding of recombinant Cj1556 to a region upstream of Cj1556. This study has identified a novel C. jejuni transcriptional regulator Cj1556 that is involved in oxidative and aerobic stress responses and is important for the survival of C. jejuni in the natural environment and in vivo. This newly identified regulator was designated CosR (Campylobacter oxidative stress Regulator). Data is also available from <ahref=""http://bugs.sgul.ac.uk/E-BUGS-119"" target=""_blank"">BuG@Sbase</a>

2012-01-23 | E-BUGS-119 | biostudies-arrayexpress

Campylobacter jejuni

Project description:Campylobacter jejuni genes contributing to acid adaptation

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Campylobacter jejuni gene expression in germ-free mice

Project description:Expression arrays comparing Campylobacter jejuni NCTC11168 during growth in the cecum of germ-free C57 BL/6 IL-10 knockout mice to C. jejuni NCTC11168 during growth in Bolton broth.

2011-04-14 | GSE28578 | GEO

Transcriptional profile in macrolide susceptible and resistant Campylobacter jejuni

Project description:Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain. C. jejuni is prevalent in chickens, a natural reservoir for this pathogenic organism. Due to the importance of macrolide antibiotics in clinical therapy of human campylobacteriosis, development of macrolide resistance in Campylobacter has become a concern for public health.To facilitate understanding the molecular basis associated with the fitness difference between Erys and Eryr Campylobacter, we compared the transcriptomes between ATCC 700819 and its isogenic Eryr transformant T.L.101 using DNA microarray.

2011-11-02 | GSE33330 | GEO

Campylobacter jejuni murine adaptation: unadapted v. adapted

Project description:Expression arrays comparing Campylobacter jejuni 11168 before and after serial passage in C57 BL/6 IL-10 deficient mice. Gene expression was compared during exponential growth in Bolton broth.

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